Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1988 Apr;8(4):1845–1848. doi: 10.1128/mcb.8.4.1845

Retinoic acid increases the sensitivity of the rat embryo fibroblast transformation assay.

T D Halazonetis 1, C Daugherty 1, P Leder 1
PMCID: PMC363352  PMID: 3380100

Abstract

The rat embryo fibroblast focus assay is used to evaluate the transforming potential of several oncogenes. The sensitivity of this assay increased fivefold when retinoic acid was added to tissue culture media. Retinoic acid probably acts by selectively inhibiting the proliferation of nontransformed cells.

Full text

PDF
1845

Images in this article

1846Image
on p.1846

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azarnia R., Dahl G., Loewenstein W. R. Cell junction and cycle AMP: III. Promotion of junctional membrane permeability and junctional membrane particles in a junction-deficient cell type. J Membr Biol. 1981;63(1-2):133–146. doi: 10.1007/BF01969454. [DOI] [PubMed] [Google Scholar]
  2. Besterman J. M., Tyrey S. J., Cragoe E. J., Jr, Cuatrecasas P. Inhibition of epidermal growth factor-induced mitogenesis by amiloride and an analog: evidence against a requirement for Na+/H+ exchange. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6762–6766. doi: 10.1073/pnas.81.21.6762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Breitman T. R., Selonick S. E., Collins S. J. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc Natl Acad Sci U S A. 1980 May;77(5):2936–2940. doi: 10.1073/pnas.77.5.2936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fitzgerald D. J., Barrett J. C., Nettesheim P. Changing responsiveness to all-trans retinoic acid of rat tracheal epithelial cells at different stages of neoplastic transformation. Carcinogenesis. 1986 Oct;7(10):1715–1721. doi: 10.1093/carcin/7.10.1715. [DOI] [PubMed] [Google Scholar]
  5. Jetten A. M., Barrett J. C., Gilmer T. M. Differential response to retinoic acid of Syrian hamster embryo fibroblasts expressing v-src or v-Ha-ras oncogenes. Mol Cell Biol. 1986 Oct;6(10):3341–3348. doi: 10.1128/mcb.6.10.3341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jetten A. M., Jetten M. E. Possible role of retinoic acid binding protein in retinoid stimulation of embryonal carcinoma cell differentiation. Nature. 1979 Mar 8;278(5700):180–182. doi: 10.1038/278180a0. [DOI] [PubMed] [Google Scholar]
  7. Jetten A. M., Jetten M. E., Shapiro S. S., Poon J. P. Characterization of the action of retinoids on mouse fibroblast cell lines. Exp Cell Res. 1979 Mar 15;119(2):289–299. doi: 10.1016/0014-4827(79)90356-2. [DOI] [PubMed] [Google Scholar]
  8. Jetten A. M. Modulation of cell growth by retinoids and their possible mechanisms of action. Fed Proc. 1984 Jan;43(1):134–139. [PubMed] [Google Scholar]
  9. Land H., Chen A. C., Morgenstern J. P., Parada L. F., Weinberg R. A. Behavior of myc and ras oncogenes in transformation of rat embryo fibroblasts. Mol Cell Biol. 1986 Jun;6(6):1917–1925. doi: 10.1128/mcb.6.6.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Land H., Parada L. F., Weinberg R. A. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature. 1983 Aug 18;304(5927):596–602. doi: 10.1038/304596a0. [DOI] [PubMed] [Google Scholar]
  11. Lotan R., Nicolson G. L. Inhibitory effects of retinoic acid or retinyl acetate on the growth of untransformed, transformed, and tumor cells in vitro. J Natl Cancer Inst. 1977 Dec;59(6):1717–1722. doi: 10.1093/jnci/59.6.1717. [DOI] [PubMed] [Google Scholar]
  12. Mehta P. P., Bertram J. S., Loewenstein W. R. Growth inhibition of transformed cells correlates with their junctional communication with normal cells. Cell. 1986 Jan 17;44(1):187–196. doi: 10.1016/0092-8674(86)90497-6. [DOI] [PubMed] [Google Scholar]
  13. Mulligan R. C., Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072–2076. doi: 10.1073/pnas.78.4.2072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Parker B. A., Stark G. R. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Virol. 1979 Aug;31(2):360–369. doi: 10.1128/jvi.31.2.360-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Patt L. M., Itaya K., Hakomori S. I. Retinol induces density-dependent growth inhibition and changes in glycolipids and LETS. Nature. 1978 Jun 1;273(5661):379–381. doi: 10.1038/273379a0. [DOI] [PubMed] [Google Scholar]
  16. Pitts J. D., Hamilton A. E., Kam E., Burk R. R., Murphy J. P. Retinoic acid inhibits junctional communication between animal cells. Carcinogenesis. 1986 Jun;7(6):1003–1010. doi: 10.1093/carcin/7.6.1003. [DOI] [PubMed] [Google Scholar]
  17. Sarid J., Halazonetis T. D., Murphy W., Leder P. Evolutionarily conserved regions of the human c-myc protein can be uncoupled from transforming activity. Proc Natl Acad Sci U S A. 1987 Jan;84(1):170–173. doi: 10.1073/pnas.84.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwab M., Varmus H. E., Bishop J. M. Human N-myc gene contributes to neoplastic transformation of mammalian cells in culture. Nature. 1985 Jul 11;316(6024):160–162. doi: 10.1038/316160a0. [DOI] [PubMed] [Google Scholar]
  19. Stone J., de Lange T., Ramsay G., Jakobovits E., Bishop J. M., Varmus H., Lee W. Definition of regions in human c-myc that are involved in transformation and nuclear localization. Mol Cell Biol. 1987 May;7(5):1697–1709. doi: 10.1128/mcb.7.5.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strickland S., Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell. 1978 Oct;15(2):393–403. doi: 10.1016/0092-8674(78)90008-9. [DOI] [PubMed] [Google Scholar]
  21. Tsao M. S., Blaisdell J., Chou B. B., Earp H. S., Grisham J. W. Inconsistent effects of all-trans retinoic acid on different clones of chemically transformed rat liver epithelial cells. Carcinogenesis. 1987 Apr;8(4):497–501. doi: 10.1093/carcin/8.4.497. [DOI] [PubMed] [Google Scholar]
  22. Wiener E. C., Loewenstein W. R. Correction of cell-cell communication defect by introduction of a protein kinase into mutant cells. 1983 Sep 29-Oct 5Nature. 305(5933):433–435. doi: 10.1038/305433a0. [DOI] [PubMed] [Google Scholar]
  23. Wälder L., Lützelschwab R. Effects of 12-O-tetradecanoylphorbol-13-acetate (TPA), retinoic acid and diazepam on intercellular communication in a monolayer of rat liver epithelial cells. Exp Cell Res. 1984 May;152(1):66–76. doi: 10.1016/0014-4827(84)90230-1. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES