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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Biochemistry. 2013 Feb 21;52(9):1547–1558. doi: 10.1021/bi301008r

Figure 4.

Figure 4

Effect of pro-cathelicidin, hCLD and LL-37 on the activity of human cathepsin L. (A) Hydrolysis of Z-Phe-Arg-AMC substrate by human liver cathepsin L (0.1 mU) in the presence of hCLD (concentration ranging from 0–1,000 nM). Human urine cystatin C at 10 nM concentration was used as a positive control. (B) Dose-dependent inhibition of cathepsin L (0.1 mU) by pro-cathelicidin, hCLD and LL-37 and human urine cystatin C. The remaining activity of cathepsin L was measured 20 minutes after the reaction and hydrolysis was initiated by the addition of the Z-Phe-Arg-AMC substrate. Each curve is the mean of three independent experiments.