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A) SDS PAGE analysis of fractions obtained from heparin column purification of arrestin. The supernatant (S), flow through (FT), washes (W1-4) and Elutions (E1-2) are shown. B) Western blot analysis of supernatant (S) and Elution1 (E1) from panel A. C) SDS PAGE analysis of gel filtration purified E1 from panel A. The overloaded lane shows a clean product. D) CD spectrum of wt-arr21-418 showing a predominance of beta-sheet and some evidence of alpha-helical character.
