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. 2013 Apr 8;139(1):19–29. doi: 10.1111/imm.12045

Figure 5.

Figure 5

Lamina propria mononuclear cells (LPMCs) from patients with Crohn's disease CD express TGR5 similarly to that in macrophages differentiated with macrophage colony-stimulating factor (M-CSF) and interferon-γ (IFNγ) (Mγ-Mϕs). (a) The mRNA level of TGR5 in LPMCs of non-irritable bowel disease (non-IBD), non-inflamed Crohn's disease (CD) and inflamed CD patients was measured by real-time quantitative PCR. The mRNA level of TGR5 was normalized to that of β-actin and is shown as the fold change based on the non-IBD, which is set to 1. Results are shown as the means ± SEM from four non-IBD, seven non-inflamed CD and seven inflamed CD samples. Statistical analysis was performed by Kruskal–Wallis one-way analysis of variance and the Bonferroni post-hoc test for multiple comparisons. *P < 0·05. (b) The mRNA level of TGR5 in CD14+-depleted LPMCs and CD14+ intestinal Mϕs from CD patients was measured by real-time quantitative PCR. The mRNA level of TGR5 was normalized to that of β-actin, and is shown as the fold change based on the mRNA levels in CD whole LPMCs before sorting. Results are shown as the means ± SEM from four CD14+-depleted LPMCs and four CD14+ intestinal Mϕs samples. Statistical analysis was performed by Kruskal–Wallis one-way analysis of variance and the Bonferroni post-hoc test for multiple comparisons. *P < 0·05.