Figure 3.

Myeloid-derived suppressor cells (MDSC) and regulatory T (Treg) cells from Rorγt^gfp/gfp mice do not present an altered recruitment to the tumour or phenotype. Splenocytes and tumour-infiltrating cells from wild-type (WT) and Rorγt^gfp/gfp mice were isolated and analysed by FACS. Frequency of Gr-1^highCD11b^high MDSC in a PI⁻ gate (a). Foxp3⁺ cells in a CD4⁺ gate (b). Percentage of CD49d^high cells in a Gr-1^high CD11b^high gate (c). Frequency of Gr-1^high CD11b⁻ neutrophils in a PI⁻ gate (d). CD39 and CTLA-4 mean fluorescence intensity in a CD4⁺ Foxp3⁺ gate in cells isolated from WT and Rorγt^gfp/gfp tumours (e). Bars show mean ± SEM.