Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1988 Jun;8(6):2604–2609. doi: 10.1128/mcb.8.6.2604

Differentiation of erythroid progenitor (CFU-E) cells from mouse fetal liver cells and murine erythroleukemia (TSA8) cells without proliferation.

T Noguchi 1, H Fukumoto 1, Y Mishina 1, M Obinata 1
PMCID: PMC363462  PMID: 3165492

Abstract

Erythropoietin (epo) appears to play a significant role in influencing the proliferation and differentiation of erythroid progenitor (CFU-E) cells. To determine the mechanism of action of epo, the effect of drugs on the in vitro colony formation of CFU-E cells induced from a novel murine erythroleukemia cell line, TSA8, was examined. While cytosine arabinoside inhibited colony formation and terminal differentiation of the CFU-E cells responding to epo, herbimycin, which is a drug that inhibits src-related phosphorylation, inhibited colony formation only. The same effect of herbimycin was observed with normal CFU-E cells from mouse fetal liver cells. These results suggest that epo induces two signals, one for proliferation and the other for differentiation, and that the two signals are not linked in erythroid progenitor cells.

Full text

PDF
2604

Images in this article

2606Image
on p.2606
2608Image
on p.2608

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  2. Dexter T. M., Spooncer E., Schofield R., Lord B. I., Simmons P. Haemopoietic stem cells and the problem of self-renewal. Blood Cells. 1984;10(2-3):315–339. [PubMed] [Google Scholar]
  3. Djaidetti M., Preisler H., Marks P. A., Rifkind R. A. Erythropoietin effects on fetal mouse erythroid cells. II. Nucleic acid synthesis and the erythropoietin-sensitive cell. J Biol Chem. 1972 Feb 10;247(3):731–735. [PubMed] [Google Scholar]
  4. Gambari R., Marks P. A., Rifkind R. A. Murine erythroleukemia cell differentiation: relationship of globin gene expression and of prolongation of G1 to inducer effects during G1/early S. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4511–4515. doi: 10.1073/pnas.76.9.4511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldwasser E. Erythropoietin and its mode of action. Blood Cells. 1984;10(2-3):147–162. [PubMed] [Google Scholar]
  6. Gusella J., Geller R., Clarke B., Weeks V., Housman D. Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis. Cell. 1976 Oct;9(2):221–229. doi: 10.1016/0092-8674(76)90113-6. [DOI] [PubMed] [Google Scholar]
  7. Hankins W. D., Schooley J., Eastment C. Erythropoietin, an autocrine regulator? Serum-free production of erythropoietin by cloned erythroid cell lines. Blood. 1986 Jul;68(1):263–268. [PubMed] [Google Scholar]
  8. Iscove N. N., Sieber F., Winterhalter K. H. Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. J Cell Physiol. 1974 Apr;83(2):309–320. doi: 10.1002/jcp.1040830218. [DOI] [PubMed] [Google Scholar]
  9. Kahn P., Adkins B., Beug H., Graf T. src- and fps-containing avian sarcoma viruses transform chicken erythroid cells. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7122–7126. doi: 10.1073/pnas.81.22.7122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kahn P., Frykberg L., Brady C., Stanley I., Beug H., Vennström B., Graf T. v-erbA cooperates with sarcoma oncogenes in leukemic cell transformation. Cell. 1986 May 9;45(3):349–356. doi: 10.1016/0092-8674(86)90320-x. [DOI] [PubMed] [Google Scholar]
  11. Krantz S. B., Goldwasser E. Specific binding of erythropoietin to spleen cells infected with the anemia strain of Friend virus. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7574–7578. doi: 10.1073/pnas.81.23.7574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee-Huang S. Cloning and expression of human erythropoietin cDNA in Escherichia coli. Proc Natl Acad Sci U S A. 1984 May;81(9):2708–2712. doi: 10.1073/pnas.81.9.2708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McDonald J. D., Lin F. K., Goldwasser E. Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene. Mol Cell Biol. 1986 Mar;6(3):842–848. doi: 10.1128/mcb.6.3.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mishina Y., Obinata M. Induction of commitment of murine erythroleukemia cells (TSA8) to CFU-E with DMSO. Exp Cell Res. 1986 Feb;162(2):319–325. doi: 10.1016/0014-4827(86)90337-x. [DOI] [PubMed] [Google Scholar]
  15. Noguchi T., Fukumoto H., Mishina Y., Obinata M. Factors controlling induction of commitment of murine erythroleukemia (TSA8) cells to CFU-E (colony forming unit-erythroid). Development. 1987 Sep;101(1):169–174. doi: 10.1242/dev.101.1.169. [DOI] [PubMed] [Google Scholar]
  16. Ogawa M., Porter P. N., Nakahata T. Renewal and commitment to differentiation of hemopoietic stem cells (an interpretive review). Blood. 1983 May;61(5):823–829. [PubMed] [Google Scholar]
  17. Ramirez F., Gambino R., Maniatis G. M., Rifkind R. A., Marks P. A., Bank A. Changes in globin messenger RNA content during erythroid cell differentiation. J Biol Chem. 1975 Aug 10;250(15):6054–6058. [PubMed] [Google Scholar]
  18. Shibuya T., Mak T. W. Isolation and induction of erythroleukemic cell lines with properties of erythroid progenitor burst-forming cell (BFU-E) and erythroid precursor cell (CFU-E). Proc Natl Acad Sci U S A. 1983 Jun;80(12):3721–3725. doi: 10.1073/pnas.80.12.3721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stephenson J. R., Axelrad A. A., McLeod D. L., Shreeve M. M. Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542–1546. doi: 10.1073/pnas.68.7.1542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Till J. E., McCulloch E. A. Hemopoietic stem cell differentiation. Biochim Biophys Acta. 1980 Nov 26;605(4):431–459. doi: 10.1016/0304-419x(80)90009-8. [DOI] [PubMed] [Google Scholar]
  21. Uehara Y., Hori M., Takeuchi T., Umezawa H. Phenotypic change from transformed to normal induced by benzoquinonoid ansamycins accompanies inactivation of p60src in rat kidney cells infected with Rous sarcoma virus. Mol Cell Biol. 1986 Jun;6(6):2198–2206. doi: 10.1128/mcb.6.6.2198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uehara Y., Hori M., Takeuchi T., Umezawa H. Screening of agents which convert 'transformed morphology' of Rous sarcoma virus-infected rat kidney cells to 'normal morphology': identification of an active agent as herbimycin and its inhibition of intracellular src kinase. Jpn J Cancer Res. 1985 Aug;76(8):672–675. [PubMed] [Google Scholar]
  23. Waneck G. L., Keyes L., Rosenberg N. Abelson virus drives the differentiation of Harvey virus-infected erythroid cells. Cell. 1986 Jan 31;44(2):337–344. doi: 10.1016/0092-8674(86)90768-3. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES