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. 2013 Feb 19;5(2):579–593. doi: 10.3390/nu5020579

Table 1.

Detailed Primers and Conditions Used for Real-Time PCR Assays.

Gene product Primer name Primer sequence Annealing temp (°C) Product size (bp)
IL-12A IL-12-R CACTCCCAAAACCTGCTGAG 60 88
IL-12-F TCTCTTCAGAAGTGCAAGGGTA
IL-18 IL-18-R CAACAAACTATTTGTCGCAGGA 60 73
IL-18-F TGCCACAAAGTTGATGCAAT
IL-21 IL-21-R AGGAAACCACCTTCCACAAA 60 68
IL-21-F GAATCACATGAAGGGCATGTT
β-Actin β-Actin-R TCTACAACGAGCTGCGAAT 60 115
β-Actin-F CAATTTCCCTCTCGGCCTCG