Abstract
Rapid adoption of new sequencing technologies means that sample QC in this workflow not only needs to have high throughput capability, but flexibility is also critical. The Agilent 2200 TapeStation system meets this demand by providing a simple to use automated electrophoresis platform with truly variable throughput capabilities, from a single sample up to a 96-well plate. The D1K ScreenTape assay on the 2200 TapeStation enables analysis of DNA fragments between 35 and 1000bp, suiting NGS library preparation QC and quantification. This study is an assessment of the performance the D1K ScreenTape assay in high-throughput DNA analysis using the 96-well sample plate capability. Sizing and quantification were assessed for accuracy and precision on a foil-sealed 96-well plate over typical and extended run times. The study concluded that the D1K ScreenTape assay is suited for accurate sizing and quantification of DNA fragments in high-throughput applications.