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. 1988 Aug;8(8):3322–3331. doi: 10.1128/mcb.8.8.3322

tRNA(Tyr) genes of Drosophila melanogaster: expression of single-copy genes studied by S1 mapping.

B Suter 1, E Kubli 1
PMCID: PMC363567  PMID: 3145409

Abstract

Six Drosophila melanogaster tRNA(Tyr) genes have been isolated and sequenced. They contained introns of different sequences and two size classes: 20 or 21 base pairs (bp) (five genes) and 113 bp (one gene). However, the sequences coding for the mature tRNA(Tyr) were identical in all six genes. The 113-bp intron-containing gene was a single-copy gene. Hence, its primary transcript could be traced by S1 mapping. The gene was turned on during embryogenesis and continually expressed to various degrees during the following developmental stages. Thus, S1 mapping is a feasible method to follow the transcriptional activity of individual genes with identical mature products, provided that their primary transcripts are unique. The six genes were organized in two clusters of three and two genes, respectively (each containing a 20- or a 21-bp intron; cytological localization, 85A), and a single-copy gene (113-bp intron; cytological localization, 28C). We show that four of the six tRNA(Tyr) genes characterized were localized in putative 5' control regions of developmentally controlled genes transcribed by polymerase II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Addison W. R., Astell C. R., Delaney A. D., Gillam I. C., Hayashi S., Miller R. C., Rajput B., Smith M., Taylor D. M., Tener G. M. The structures of genes hybridizing with tRNA4Val from Drosophila melanogaster. J Biol Chem. 1982 Jan 25;257(2):670–673. [PubMed] [Google Scholar]
  2. Ali M., Vedeckis W. V. The glucocorticoid receptor protein binds to transfer RNA. Science. 1987 Jan 23;235(4787):467–470. doi: 10.1126/science.3798121. [DOI] [PubMed] [Google Scholar]
  3. Altwegg M., Kubli E. The nucleotide sequence of phenylalanine tRNA2 of Drosophila melanogaster: four isoacceptors with one basic sequence. Nucleic Acids Res. 1979 Sep 11;7(1):93–105. doi: 10.1093/nar/7.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Amstutz H., Munz P., Heyer W. D., Leupoid U., Kohli J. Concerted evolution of tRNA genes: intergenic conversion among three unlinked serine tRNA genes in S. pombe. Cell. 1985 Apr;40(4):879–886. doi: 10.1016/0092-8674(85)90347-2. [DOI] [PubMed] [Google Scholar]
  5. Buck M., Ames B. N. A modified nucleotide in tRNA as a possible regulator of aerobiosis: synthesis of cis-2-methyl-thioribosylzeatin in the tRNA of Salmonella. Cell. 1984 Feb;36(2):523–531. doi: 10.1016/0092-8674(84)90245-9. [DOI] [PubMed] [Google Scholar]
  6. Choffat Y., Suter B., Behra R., Kubli E. Pseudouridine modification in the tRNA(Tyr) anticodon is dependent on the presence, but independent of the size and sequence, of the intron in eucaryotic tRNA(Tyr) genes. Mol Cell Biol. 1988 Aug;8(8):3332–3337. doi: 10.1128/mcb.8.8.3332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cribbs D. L., Leung J., Newton C. H., Hayashi S., Miller R. C., Jr, Tener G. M. Extensive microheterogeneity of serine tRNA genes from Drosophila melanogaster. J Mol Biol. 1987 Oct 5;197(3):397–404. doi: 10.1016/0022-2836(87)90553-5. [DOI] [PubMed] [Google Scholar]
  9. Dudler R., Schmidt T., Bienz M., Kubli E. The genes coding for tRNA Tyr of Drosophila melanogaster: localization of determination of the gene numbers. Chromosoma. 1981;84(1):49–60. doi: 10.1007/BF00293362. [DOI] [PubMed] [Google Scholar]
  10. Dudler R., Travers A. A. Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies. Cell. 1984 Sep;38(2):391–398. doi: 10.1016/0092-8674(84)90494-x. [DOI] [PubMed] [Google Scholar]
  11. Ferber S., Ciechanover A. Role of arginine-tRNA in protein degradation by the ubiquitin pathway. Nature. 1987 Apr 23;326(6115):808–811. doi: 10.1038/326808a0. [DOI] [PubMed] [Google Scholar]
  12. Grigliatti T. A., White B. N., Tener G. M., Kaufman T. C., Holden J. J., Suzuki D. T. Studies on the transfer RNA genes of Drosophila. Cold Spring Harb Symp Quant Biol. 1974;38:461–474. doi: 10.1101/sqb.1974.038.01.050. [DOI] [PubMed] [Google Scholar]
  13. Hayashi S., Gillam I. C., Grigliatti T. A., Tener G. M. Localization of tRNA genes of Drosophila melanogaster by in situ hybridization. Chromosoma. 1982;86(2):279–292. doi: 10.1007/BF00288682. [DOI] [PubMed] [Google Scholar]
  14. Hosbach H. A., Silberklang M., McCarthy B. J. Evolution of a D. melanogaster glutamate tRNA gene cluster. Cell. 1980 Aug;21(1):169–178. doi: 10.1016/0092-8674(80)90124-5. [DOI] [PubMed] [Google Scholar]
  15. Howes N. K., Farkas W. R. Studies with a homogeneous enzyme from rabbit erythrocytes catalyzing the insertion of guanine into tRNA. J Biol Chem. 1978 Dec 25;253(24):9082–9087. [PubMed] [Google Scholar]
  16. Johnson P. F., Abelson J. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product. Nature. 1983 Apr 21;302(5910):681–687. doi: 10.1038/302681a0. [DOI] [PubMed] [Google Scholar]
  17. Kubli E. The genetics of transfer RNA in Drosophila. Adv Genet. 1982;21:123–172. doi: 10.1016/s0065-2660(08)60298-9. [DOI] [PubMed] [Google Scholar]
  18. Meier F., Suter B., Grosjean H., Keith G., Kubli E. Queuosine modification of the wobble base in tRNAHis influences 'in vivo' decoding properties. EMBO J. 1985 Mar;4(3):823–827. doi: 10.1002/j.1460-2075.1985.tb03704.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ogden R. C., Lee M. C., Knapp G. Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates. Nucleic Acids Res. 1984 Dec 21;12(24):9367–9382. doi: 10.1093/nar/12.24.9367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pardue M. L., Gall J. G. Nucleic acid hybridization to the DNA of cytological preparations. Methods Cell Biol. 1975;10:1–16. doi: 10.1016/s0091-679x(08)60727-x. [DOI] [PubMed] [Google Scholar]
  21. Picard D., Schaffner W. Correct transcription of a cloned mouse immunoglobulin gene in vivo. Proc Natl Acad Sci U S A. 1983 Jan;80(2):417–421. doi: 10.1073/pnas.80.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Saluz H. P., Schmidt T., Dudler R., Altwegg M., Stumm-Zollinger E., Kubli E., Chen P. S. The genes coding for 4 snRNAs of Drosophila melanogaster: localization and determination of gene numbers. Nucleic Acids Res. 1983 Jan 11;11(1):77–90. doi: 10.1093/nar/11.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schön A., Krupp G., Gough S., Berry-Lowe S., Kannangara C. G., Söll D. The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA. Nature. 1986 Jul 17;322(6076):281–284. doi: 10.1038/322281a0. [DOI] [PubMed] [Google Scholar]
  25. Sharp S. J., Schaack J., Cooley L., Burke D. J., Söll D. Structure and transcription of eukaryotic tRNA genes. CRC Crit Rev Biochem. 1985;19(2):107–144. doi: 10.3109/10409238509082541. [DOI] [PubMed] [Google Scholar]
  26. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  27. Suter B., Altwegg M., Choffat Y., Kubli E. The nucleotide sequence of two homogeneic Drosophila melanogaster tRNATyr isoacceptors: application of a rapid tRNA anticodon sequencing method using S-1 nuclease. Arch Biochem Biophys. 1986 May 15;247(1):233–237. doi: 10.1016/0003-9861(86)90552-7. [DOI] [PubMed] [Google Scholar]
  28. Treisman R., Proudfoot N. J., Shander M., Maniatis T. A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing. Cell. 1982 Jul;29(3):903–911. doi: 10.1016/0092-8674(82)90452-4. [DOI] [PubMed] [Google Scholar]
  29. Wittig B., Wittig S. Purification of class A, B, and C DNA-dependent RNA polymerases from chicken embryos. Biochim Biophys Acta. 1978 Oct 24;520(3):598–611. doi: 10.1016/0005-2787(78)90145-4. [DOI] [PubMed] [Google Scholar]
  30. Woo S. L. A sensitive and rapid method for recombinant phage screening. Methods Enzymol. 1979;68:389–395. doi: 10.1016/0076-6879(79)68028-x. [DOI] [PubMed] [Google Scholar]

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