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. 1989 Nov;9(11):4819–4823. doi: 10.1128/mcb.9.11.4819

Characterization of phytochrome-regulated gene expression in a photoautotrophic cell suspension: possible role for calmodulin.

E Lam 1, M Benedyk 1, N H Chua 1
PMCID: PMC363631  PMID: 2689868

Abstract

A photoautotrophic suspension culture of soybeans was found to exhibit light-dependent expression of the genes encoding the major chlorophyll a- and b-binding protein (CAB). The expression was mediated by phytochrome, since it was induced by red light and reversed by far-red light. The maximal level as well as the kinetics of the induction were comparable between the suspension culture and soybean seedlings. Using this cell culture, we addressed the question of whether a calcium- and/or calmodulin-mediated step is involved in the signal transduction process between phytochrome and CAB expression. We found that W-7, a potent calmodulin antagonist, severely attenuated the induction of CAB mRNA by light, whereas W-5, a weak calmodulin antagonist, had little effect. Control experiments demonstrated that W-7 treatment did not block the induction of hsp-75 by heat shock. The addition of ionomycin, a calcium ionophore, induced a low level of CAB mRNA accumulation in the dark which could be further enhanced by light treatment. We propose that calmodulin activation by light is necessary but not sufficient to induce maximal CAB expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benfey P. N., Chua N. H. Regulated genes in transgenic plants. Science. 1989 Apr 14;244(4901):174–181. doi: 10.1126/science.244.4901.174. [DOI] [PubMed] [Google Scholar]
  2. Datta N., Chen Y. R., Roux S. J. Phytochrome and calcium stimulation of protein phosphorylation in isolated pea nuclei. Biochem Biophys Res Commun. 1985 May 16;128(3):1403–1408. doi: 10.1016/0006-291x(85)91096-4. [DOI] [PubMed] [Google Scholar]
  3. Gilman A. G. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615–649. doi: 10.1146/annurev.bi.56.070187.003151. [DOI] [PubMed] [Google Scholar]
  4. Giuliano G., Pichersky E., Malik V. S., Timko M. P., Scolnik P. A., Cashmore A. R. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089–7093. doi: 10.1073/pnas.85.19.7089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green P. J., Kay S. A., Chua N. H. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J. 1987 Sep;6(9):2543–2549. doi: 10.1002/j.1460-2075.1987.tb02542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hidaka H., Sasaki Y., Tanaka T., Endo T., Ohno S., Fujii Y., Nagata T. N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4354–4357. doi: 10.1073/pnas.78.7.4354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Horn M. E., Sherrard J. H., Widholm J. M. Photoautotrophic growth of soybean cells in suspension culture: I. Establishment of photoautotrophic cultures. Plant Physiol. 1983 Jun;72(2):426–429. doi: 10.1104/pp.72.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaufman L. S., Thompson W. F., Briggs W. R. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA. Science. 1984 Dec 21;226(4681):1447–1449. doi: 10.1126/science.226.4681.1447. [DOI] [PubMed] [Google Scholar]
  9. Kuhlemeier C., Cuozzo M., Green P. J., Goyvaerts E., Ward K., Chua N. H. Localization and conditional redundancy of regulatory elements in rbcS-3A, a pea gene encoding the small subunit of ribulose-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4662–4666. doi: 10.1073/pnas.85.13.4662. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mathis J. N., Burkey K. O. Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings : a species comparison. Plant Physiol. 1987 Dec;85(4):971–977. doi: 10.1104/pp.85.4.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagy F., Boutry M., Hsu M. Y., Wong M., Chua N. H. The 5'-proximal region of the wheat Cab-1 gene contains a 268-bp enhancer-like sequence for phytochrome response. EMBO J. 1987 Sep;6(9):2537–2542. doi: 10.1002/j.1460-2075.1987.tb02541.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nagy F., Morelli G., Fraley R. T., Rogers S. G., Chua N. H. Photoregulated expression of a pea rbcS gene in leaves of transgenic plants. EMBO J. 1985 Dec 1;4(12):3063–3068. doi: 10.1002/j.1460-2075.1985.tb04046.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nishizuka Y. Studies and perspectives of protein kinase C. Science. 1986 Jul 18;233(4761):305–312. doi: 10.1126/science.3014651. [DOI] [PubMed] [Google Scholar]
  14. Paulsen H., Bogorad L. Diurnal and Circadian Rhythms in the Accumulation and Synthesis of mRNA for the Light-Harvesting Chlorophyll a/b-Binding Protein in Tobacco. Plant Physiol. 1988 Dec;88(4):1104–1109. doi: 10.1104/pp.88.4.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roux S. J., McEntire K., Slocum R. D., Cedel T. E., Hale C. C. Phytochrome induces photoreversible calcium fluxes in a purified mitochondrial fraction from oats. Proc Natl Acad Sci U S A. 1981 Jan;78(1):283–287. doi: 10.1073/pnas.78.1.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Walling L., Drews G. N., Goldberg R. B. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123–2127. doi: 10.1073/pnas.83.7.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]

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