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. 1989 Nov;9(11):5231–5233. doi: 10.1128/mcb.9.11.5231

A gamma interferon-unresponsive variant of cell line 70Z/3, IFN-4, can be partially rescued by phorbol myristate acetate.

D W Emery 1, J W Rooney 1, C H Sibley 1
PMCID: PMC363678  PMID: 2513484

Abstract

Lipopolysaccharide (LPS) and gamma interferon (IFN-gamma) induce kappa transcription in 70Z/3 cells by different mechanisms; LPS treatment induces both NF-kappa B and OTF-2 transcription factors, but IFN-gamma treatment does not. We have dissected these two activation pathways by selecting and analyzing an LPS+ IFN- variant of 70Z/3.

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Selected References

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  1. Anderson W. B., Estival A., Tapiovaara H., Gopalakrishna R. Altered subcellular distribution of protein kinase C (a phorbol ester receptor). Possible role in tumor promotion and the regulation of cell growth: relationship to changes in adenylate cyclase activity. Adv Cyclic Nucleotide Protein Phosphorylation Res. 1985;19:287–306. [PubMed] [Google Scholar]
  2. Atchison M. L., Perry R. P. Complementation between two cell lines lacking kappa enhancer activity: implications for the developmental control of immunoglobulin transcription. EMBO J. 1988 Dec 20;7(13):4213–4220. doi: 10.1002/j.1460-2075.1988.tb03318.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atchison M. L., Perry R. P. The role of the kappa enhancer and its binding factor NF-kappa B in the developmental regulation of kappa gene transcription. Cell. 1987 Jan 16;48(1):121–128. doi: 10.1016/0092-8674(87)90362-x. [DOI] [PubMed] [Google Scholar]
  4. Baeuerle P. A., Baltimore D. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor. Cell. 1988 Apr 22;53(2):211–217. doi: 10.1016/0092-8674(88)90382-0. [DOI] [PubMed] [Google Scholar]
  5. Briskin M., Kuwabara M. D., Sigman D. S., Wall R. Induction of kappa transcription by interferon-gamma without activation of NF-kappa B. Science. 1988 Nov 18;242(4881):1036–1037. doi: 10.1126/science.3143155. [DOI] [PubMed] [Google Scholar]
  6. Dignam J. D., Lebovitz R. M., Roeder R. G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983 Mar 11;11(5):1475–1489. doi: 10.1093/nar/11.5.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ephrussi A., Church G. M., Tonegawa S., Gilbert W. B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo. Science. 1985 Jan 11;227(4683):134–140. doi: 10.1126/science.3917574. [DOI] [PubMed] [Google Scholar]
  8. Fried M., Crothers D. M. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 1981 Dec 11;9(23):6505–6525. doi: 10.1093/nar/9.23.6505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mains P. E., Sibley C. H. LPS-nonresponsive variants of mouse B cell lymphoma, 70Z/3: isolation and characterization. Somatic Cell Genet. 1983 Nov;9(6):699–720. doi: 10.1007/BF01539475. [DOI] [PubMed] [Google Scholar]
  10. Mather E. L. DNA-binding factors of B lymphoid cells are susceptible to limited proteolytic cleavage during nuclear extract preparation. Mol Cell Biol. 1988 Apr;8(4):1812–1815. doi: 10.1128/mcb.8.4.1812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Müller M. M., Gerster T., Schaffner W. Enhancer sequences and the regulation of gene transcription. Eur J Biochem. 1988 Oct 1;176(3):485–495. doi: 10.1111/j.1432-1033.1988.tb14306.x. [DOI] [PubMed] [Google Scholar]
  12. Paige C. J., Kincade P. W., Ralph P. Independent control of immunoglobulin heavy and light chain expression in a murine pre-B-cell line. Nature. 1981 Aug 13;292(5824):631–633. doi: 10.1038/292631a0. [DOI] [PubMed] [Google Scholar]
  13. Parslow T. G., Blair D. L., Murphy W. J., Granner D. K. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence. Proc Natl Acad Sci U S A. 1984 May;81(9):2650–2654. doi: 10.1073/pnas.81.9.2650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Picard D., Schaffner W. A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene. Nature. 1984 Jan 5;307(5946):80–82. doi: 10.1038/307080a0. [DOI] [PubMed] [Google Scholar]
  15. Queen C., Baltimore D. Immunoglobulin gene transcription is activated by downstream sequence elements. Cell. 1983 Jul;33(3):741–748. doi: 10.1016/0092-8674(83)90016-8. [DOI] [PubMed] [Google Scholar]
  16. Queen C., Stafford J. Fine mapping of an immunoglobulin gene activator. Mol Cell Biol. 1984 Jun;4(6):1042–1049. doi: 10.1128/mcb.4.6.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sen R., Baltimore D. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism. Cell. 1986 Dec 26;47(6):921–928. doi: 10.1016/0092-8674(86)90807-x. [DOI] [PubMed] [Google Scholar]
  18. Sen R., Baltimore D. Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell. 1986 Aug 29;46(5):705–716. doi: 10.1016/0092-8674(86)90346-6. [DOI] [PubMed] [Google Scholar]
  19. Singh H., Sen R., Baltimore D., Sharp P. A. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature. 1986 Jan 9;319(6049):154–158. doi: 10.1038/319154a0. [DOI] [PubMed] [Google Scholar]
  20. Staudt L. M., Clerc R. G., Singh H., LeBowitz J. H., Sharp P. A., Baltimore D. Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif. Science. 1988 Jul 29;241(4865):577–580. doi: 10.1126/science.3399892. [DOI] [PubMed] [Google Scholar]
  21. Stimac E., Lyons S., Pious D. Transcription of HLA class II genes in the absence of B-cell-specific octamer-binding factor. Mol Cell Biol. 1988 Sep;8(9):3734–3739. doi: 10.1128/mcb.8.9.3734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Weeks R. S., Sibley C. H. Inducible expression of transfected kappa light chains by lipopolysaccharide and IFN-gamma in the murine B lymphoma, 70Z/3. J Immunol. 1988 Feb 15;140(4):1312–1320. [PubMed] [Google Scholar]
  23. Weeks R. S., Sibley C. H. Molecular analysis of immunoglobulin expression in variants of murine B lymphoma, 70Z/3. Somat Cell Mol Genet. 1987 May;13(3):205–219. doi: 10.1007/BF01535203. [DOI] [PubMed] [Google Scholar]

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