Abstract
We have previously shown that transcription from a Xenopus 5S rRNA gene assembled into chromatin in vitro can be repressed in the absence of histone H1 at high nucleosome densities (one nucleosome per 160 base pairs of DNA) (A. Shimamura, D. Tremethick, and A. Worcel, Mol. Cell. Biol. 8:4257-4269, 1988). We report here that transcriptional repression may also be achieved at lower nucleosome densities (one nucleosome per 215 base pairs of DNA) when histone H1 is present. Removal of histone H1 from the minichromosomes with Biorex under conditions in which no nucleosome disruption was observed led to transcriptional activation. Transcriptional repression could be restored by adding histone H1 back to the H1-depleted minichromosomes. The levels of histone H1 that repressed the H1-depleted minichromosomes failed to repress transcription from free DNA templates present in trans. The assembly of transcription complexes onto the H1-depleted minichromosomes protected the 5S RNA gene from inactivation by histone H1.
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Selected References
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- Allan J., Cowling G. J., Harborne N., Cattini P., Craigie R., Gould H. Regulation of the higher-order structure of chromatin by histones H1 and H5. J Cell Biol. 1981 Aug;90(2):279–288. doi: 10.1083/jcb.90.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Almer A., Rudolph H., Hinnen A., Hörz W. Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements. EMBO J. 1986 Oct;5(10):2689–2696. doi: 10.1002/j.1460-2075.1986.tb04552.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Billett M. A., Hall T. J. Cations and the accessibility of chromatin to nucleases. Nucleic Acids Res. 1979 Jun 25;6(8):2929–2945. doi: 10.1093/nar/6.8.2929. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bogenhagen D. F., Sakonju S., Brown D. D. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. Cell. 1980 Jan;19(1):27–35. doi: 10.1016/0092-8674(80)90385-2. [DOI] [PubMed] [Google Scholar]
- Bogenhagen D. F., Wormington W. M., Brown D. D. Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state. Cell. 1982 Feb;28(2):413–421. doi: 10.1016/0092-8674(82)90359-2. [DOI] [PubMed] [Google Scholar]
- Brown D. D. The role of stable complexes that repress and activate eucaryotic genes. Cell. 1984 Jun;37(2):359–365. doi: 10.1016/0092-8674(84)90366-0. [DOI] [PubMed] [Google Scholar]
- Dignam J. D., Martin P. L., Shastry B. S., Roeder R. G. Eukaryotic gene transcription with purified components. Methods Enzymol. 1983;101:582–598. doi: 10.1016/0076-6879(83)01039-3. [DOI] [PubMed] [Google Scholar]
- Dilworth S. M., Black S. J., Laskey R. A. Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts. Cell. 1987 Dec 24;51(6):1009–1018. doi: 10.1016/0092-8674(87)90587-3. [DOI] [PubMed] [Google Scholar]
- Engelke D. R., Ng S. Y., Shastry B. S., Roeder R. G. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. Cell. 1980 Mar;19(3):717–728. doi: 10.1016/s0092-8674(80)80048-1. [DOI] [PubMed] [Google Scholar]
- Fisher E. A., Felsenfeld G. Comparison of the folding of beta-globin and ovalbumin gene containing chromatin isolated from chicken oviduct and erythrocytes. Biochemistry. 1986 Dec 2;25(24):8010–8016. doi: 10.1021/bi00372a033. [DOI] [PubMed] [Google Scholar]
- Fulmer A. W., Fasman G. D. Analysis of chromatin reconstitutiion. Biochemistry. 1979 Feb 20;18(4):659–668. doi: 10.1021/bi00571a017. [DOI] [PubMed] [Google Scholar]
- Glikin G. C., Ruberti I., Worcel A. Chromatin assembly in Xenopus oocytes: in vitro studies. Cell. 1984 May;37(1):33–41. doi: 10.1016/0092-8674(84)90298-8. [DOI] [PubMed] [Google Scholar]
- Gottesfeld J., Bloomer L. S. Assembly of transcriptionally active 5S RNA gene chromatin in vitro. Cell. 1982 Apr;28(4):781–791. doi: 10.1016/0092-8674(82)90057-5. [DOI] [PubMed] [Google Scholar]
- Graziano V., Gerchman S. E., Ramakrishnan V. Reconstitution of chromatin higher-order structure from histone H5 and depleted chromatin. J Mol Biol. 1988 Oct 20;203(4):997–1007. doi: 10.1016/0022-2836(88)90124-6. [DOI] [PubMed] [Google Scholar]
- Hannon R., Bateman E., Allan J., Harborne N., Gould H. Control of RNA polymerase binding to chromatin by variations in linker histone composition. J Mol Biol. 1984 Nov 25;180(1):131–149. doi: 10.1016/0022-2836(84)90434-0. [DOI] [PubMed] [Google Scholar]
- Ilyin Y. V., Varshavsky A. Y., Mickelsaar U. N., Georgiev G. P. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA. Eur J Biochem. 1971 Sep 24;22(2):235–245. doi: 10.1111/j.1432-1033.1971.tb01537.x. [DOI] [PubMed] [Google Scholar]
- Kimura T., Mills F. C., Allan J., Gould H. Selective unfolding of erythroid chromatin in the region of the active beta-globin gene. Nature. 1983 Dec 15;306(5944):709–712. doi: 10.1038/306709a0. [DOI] [PubMed] [Google Scholar]
- Kleinschmidt J. A., Fortkamp E., Krohne G., Zentgraf H., Franke W. W. Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes. J Biol Chem. 1985 Jan 25;260(2):1166–1176. [PubMed] [Google Scholar]
- Kleinschmidt J. A., Franke W. W. Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes. Cell. 1982 Jul;29(3):799–809. doi: 10.1016/0092-8674(82)90442-1. [DOI] [PubMed] [Google Scholar]
- Knezetic J. A., Jacob G. A., Luse D. S. Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates. Mol Cell Biol. 1988 Aug;8(8):3114–3121. doi: 10.1128/mcb.8.8.3114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knezetic J. A., Luse D. S. The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro. Cell. 1986 Apr 11;45(1):95–104. doi: 10.1016/0092-8674(86)90541-6. [DOI] [PubMed] [Google Scholar]
- Künzler P., Stein A. Histone H5 can increase the internucleosome spacing in dinucleosomes to nativelike values. Biochemistry. 1983 Apr 12;22(8):1783–1789. doi: 10.1021/bi00277a007. [DOI] [PubMed] [Google Scholar]
- Laskey R. A., Honda B. M., Mills A. D., Finch J. T. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA. Nature. 1978 Oct 5;275(5679):416–420. doi: 10.1038/275416a0. [DOI] [PubMed] [Google Scholar]
- Marekov L. N., Beltchev B. Selective removal of histone H1 from chromatin at low salt concentration. Anal Biochem. 1981 Jul 15;115(1):93–96. doi: 10.1016/0003-2697(81)90529-7. [DOI] [PubMed] [Google Scholar]
- Matsui T. Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly. Mol Cell Biol. 1987 Apr;7(4):1401–1408. doi: 10.1128/mcb.7.4.1401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noll M., Kornberg R. D. Action of micrococcal nuclease on chromatin and the location of histone H1. J Mol Biol. 1977 Jan 25;109(3):393–404. doi: 10.1016/s0022-2836(77)80019-3. [DOI] [PubMed] [Google Scholar]
- Oddou P., Schmidt U., Knippers R., Richter A. Monoclonal antibodies neutralizing mammalian DNA topoisomerase I activity. Eur J Biochem. 1988 Nov 15;177(3):523–529. doi: 10.1111/j.1432-1033.1988.tb14404.x. [DOI] [PubMed] [Google Scholar]
- Razvi F., Gargiulo G., Worcel A. A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA. Gene. 1983 Aug;23(2):175–183. doi: 10.1016/0378-1119(83)90049-5. [DOI] [PubMed] [Google Scholar]
- Richard-Foy H., Hager G. L. Sequence-specific positioning of nucleosomes over the steroid-inducible MMTV promoter. EMBO J. 1987 Aug;6(8):2321–2328. doi: 10.1002/j.1460-2075.1987.tb02507.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rodríguez-Campos A., Shimamura A., Worcel A. Assembly and properties of chromatin containing histone H1. J Mol Biol. 1989 Sep 5;209(1):135–150. doi: 10.1016/0022-2836(89)90177-0. [DOI] [PubMed] [Google Scholar]
- Ruberti I., Worcel A. Mechanism of chromatin assembly in Xenopus oocytes. J Mol Biol. 1986 Jun 5;189(3):457–476. doi: 10.1016/0022-2836(86)90317-7. [DOI] [PubMed] [Google Scholar]
- Ruiz-Carrillo A., Jorcano J. L., Eder G., Lurz R. In vitro core particle and nucleosome assembly at physiological ionic strength. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3284–3288. doi: 10.1073/pnas.76.7.3284. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakonju S., Bogenhagen D. F., Brown D. D. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980 Jan;19(1):13–25. doi: 10.1016/0092-8674(80)90384-0. [DOI] [PubMed] [Google Scholar]
- Schlissel M. S., Brown D. D. The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1. Cell. 1984 Jul;37(3):903–913. doi: 10.1016/0092-8674(84)90425-2. [DOI] [PubMed] [Google Scholar]
- Shastry B. S., Ng S. Y., Roeder R. G. Multiple factors involved in the transcription of class III genes in Xenopus laevis. J Biol Chem. 1982 Nov 10;257(21):12979–12986. [PubMed] [Google Scholar]
- Shimamura A., Tremethick D., Worcel A. Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes. Mol Cell Biol. 1988 Oct;8(10):4257–4269. doi: 10.1128/mcb.8.10.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. D., Yu J., Annunziato A., Seale R. L. beta-Globin gene family in murine erythroleukemia cells resides within two chromatin domains differing in higher order structure. Biochemistry. 1984 Jun 19;23(13):2970–2976. doi: 10.1021/bi00308a019. [DOI] [PubMed] [Google Scholar]
- Stein A., Bina M. A model chromatin assembly system. Factors affecting nucleosome spacing. J Mol Biol. 1984 Sep 15;178(2):341–363. doi: 10.1016/0022-2836(84)90148-7. [DOI] [PubMed] [Google Scholar]
- Stein A., Mitchell M. Generation of different nucleosome spacing periodicities in vitro. Possible origin of cell type specificity. J Mol Biol. 1988 Oct 20;203(4):1029–1043. doi: 10.1016/0022-2836(88)90127-1. [DOI] [PubMed] [Google Scholar]
- Thoma F., Koller T., Klug A. Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin. J Cell Biol. 1979 Nov;83(2 Pt 1):403–427. doi: 10.1083/jcb.83.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thoma F., Koller T. Unravelled nucleosomes, nucleosome beads and higher order structures of chromatin: influence of non-histone components and histone H1. J Mol Biol. 1981 Jul 15;149(4):709–733. doi: 10.1016/0022-2836(81)90354-5. [DOI] [PubMed] [Google Scholar]
- Weintraub H. Histone-H1-dependent chromatin superstructures and the suppression of gene activity. Cell. 1984 Aug;38(1):17–27. doi: 10.1016/0092-8674(84)90522-1. [DOI] [PubMed] [Google Scholar]
- Wolffe A. P. Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1. EMBO J. 1989 Feb;8(2):527–537. doi: 10.1002/j.1460-2075.1989.tb03407.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolffe A. P. Transcriptional activation of Xenopus class III genes in chromatin isolated from sperm and somatic nuclei. Nucleic Acids Res. 1989 Jan 25;17(2):767–780. doi: 10.1093/nar/17.2.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Workman J. L., Abmayr S. M., Cromlish W. A., Roeder R. G. Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly. Cell. 1988 Oct 21;55(2):211–219. doi: 10.1016/0092-8674(88)90044-x. [DOI] [PubMed] [Google Scholar]
- Workman J. L., Roeder R. G. Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II. Cell. 1987 Nov 20;51(4):613–622. doi: 10.1016/0092-8674(87)90130-9. [DOI] [PubMed] [Google Scholar]
- Xing Y. Y., Worcel A. The C-terminal domain of transcription factor IIIA interacts differently with different 5S RNA genes. Mol Cell Biol. 1989 Feb;9(2):499–514. doi: 10.1128/mcb.9.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]