Figure 4.

INZ and DOX demonstrate synergistic cytotoxicity in H460 and HCT116^p53+/+ cells. H460 (A) and HCT116^p53+/+ (B) cells were plated in 96-well plates and treated with INZ or DOX individually at serial dilutions or both simultaneously at fixed molar drug ratios (INZ/DOX = 20:1 for H460 and HCT116^p53+/+) for 72 hours. Cell viability was determined using a WST cell growth assay. The data here represent the mean of three independent experiments ± SEM. CI values presented indicate the synergy of INZ with DOX assessed as that for INZ and CIS inFigure 3.