Table 1. C. elegans strain description, homology, and lifespan.
The role of in vivo oxidant stress on longevity was studied in three RC subunit missense mutants, three MnSOD knockout mutants, and an insulin receptor missense mutant. FUDR-based median lifespan assessment was largely consistent with previously reported lifespan findings in these strains. * indicates concurrent wild-type (N2 Bristol) control for MnSOD mutant lifespan comparison. All other strains were concurrently studied with the unmarked wild-type (N2 Bristol) control. RC, respiratory chain. Lifespan plots and animal number studied for each mutant are provided in Supplementary Figure 4.
| C. elegans Strain Name | Gene Function and Human Homologue | Mutation Type | Human-Worm Protein Similarity | Lifespan (20°C) | |||
|---|---|---|---|---|---|---|---|
| Interpretation | Median | Mean | Maximum | ||||
| N2 Bristol | - | wild-type | - | vs. RC Mutants vs. MnSOD mutants* |
14 17 |
16.9 19.9 |
21 23 |
| gas-1 (fc21) | Complex I subunit (NDUFS2) | missense | 83.4% | short-lived | 12 | 15.6 | 20 |
| mev-1 (kn1) | Complex II subunit (SDH-C) | missense | 75.3% | unchanged | 14 | 16.4 | 22 |
| isp-1 (qm150) | Complex III subunit (UQCRFS1) | missense | 97.5% | long-lived | 20 | 22.4 | 31 |
| daf-2 (e1368) | Insulin receptor (IR) | missense | 62.0% | long-lived | 31 | 31.1 | 38 |
| sod-2 (gk257) | Mitochondrial MnSOD (SOD2) - Cel chr. X | knockout | 97.3% | long-lived* | 19 | 20.7 | 24 |
| sod-2 (ok1030) | Mitochondrial MnSOD (SOD2) - Cel chr. X | knockout | 97.3% | long-lived* | 20 | 21.6 | 25 |
| sod-3 (gk235) | Mitochondrial MnSOD (SOD2) - Cel chr. I | knockout | 97.2% | long-lived* | 18.5 | 20.0 | 23 |