Abstract
Various pathological conditions are associated with changes in multiple protein biomarkers, and these changes can be assessed using xMAP™ beads and the Luminex™ platform. Although this platform is most commonly utilized in the analysis of biomarkers in serum, plasma, or urine, it is often desirable to measure these analytes in tissues (e.g., biopsy specimens). We have developed a simple, rapid method of tissue isolation in which excised tissues are permeabilized in RNAlater™ at 4°C overnight, followed by Luminex™ analysis for inflammatory biomarkers (cytokines). This method was compared with flash‐freezing in both mouse liver and human debrided wound specimens, and may be of utility in other clinical settings. J. Clin. Lab. Anal. 22:278–281, 2008. © 2008 Wiley‐Liss, Inc.
Keywords: Luminex™, cytokine, chemokine, biomarker, mouse, human
| Abbreviations | |
|---|---|
| FGF | fibroblast growth factor |
| IL | interleukin |
| IP‐10 | interferon‐γ‐inducible protein, 10 kDa |
| KC | keratinocyte‐derived chemokine |
| LPS | bacterial lipopolysaccharide |
| MIG | macrophage‐inducible gene. |
REFERENCES
- 1. Moseley FL, Bicknell KA, Marber MS, Brooks G. The use of proteomics to identify novel therapeutic targets for the treatment of disease. J Pharm Pharmacol 2007;59:609–628. [DOI] [PubMed] [Google Scholar]
- 2. Lv LL, Liu BC. High‐throughput antibody microarrays for quantitative proteomic analysis. Expert Rev Proteomics 2007;4:505–513. [DOI] [PubMed] [Google Scholar]
- 3. Cho WC. Proteomics technologies and challenges. Genomics proteomics. Bioinformatics 2007;5:77–85. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4. Vignali DA. Multiplexed particle‐based flow cytometric assays. J Immunol Methods 2000;243:243–255. [DOI] [PubMed] [Google Scholar]
- 5. Olle EW, Ren X, McClintock SD, et al. Matrix metalloproteinase‐9 is an important factor in hepatic regeneration after partial hepatectomy in mice. Hepatology 2006;44:540–549. [DOI] [PubMed] [Google Scholar]
- 6. RNAlater® Tissue Collection : RNA Stabilization Solution. Austin, TX: Ambion, Inc., 2007.
- 7. Jarrar D, Chaudry IH, Wang P. Organ dysfunction following hemorrhage and sepsis: Mechanisms and therapeutic approaches (review). Int J Mol Med 1999;4:575–583. [DOI] [PubMed] [Google Scholar]
- 8. Hart J. Inflammation. 1: Its role in the healing of acute wounds. J Wound Care 2002;11:205–209. [DOI] [PubMed] [Google Scholar]
- 9. Hart J. Inflammation. 2: Its role in the healing of chronic wounds. J Wound Care 2002;11:245–249. [DOI] [PubMed] [Google Scholar]
- 10. Lauta VM. A review of the cytokine network in multiple myeloma: Diagnostic, prognostic, and therapeutic implications. Cancer 2003;97:2440–2452. [DOI] [PubMed] [Google Scholar]
- 11. Blake GJ. Inflammatory biomarkers of the patient with myocardial insufficiency. Curr Opin Crit Care 2003;9:369–374. [DOI] [PubMed] [Google Scholar]
- 12. Thavasu PW, Longhurst S, Joel SP, Slevin ML, Balkwill FR. Measuring cytokine levels in blood. Importance of anticoagulants, processing, and storage conditions. J Immunol Methods 1992;153:115–124. [DOI] [PubMed] [Google Scholar]