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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5540–5552. doi: 10.1021/bi100071j

Figure 5.

Figure 5

(A–B) Representative plots for binding site size determination of intercalator-neomycin conjugates with poly(dA)•2poly(dT) as determined by UV thermal denaturation. (C) Plot for determination of binding site determination of Poly(dA)•2poly(dT) and naphthalene diimide. Graph shows the change in fluorescence of naphthalenedimide when titrated with Poly(dA)•2poly(dT). Naphthalenedimide was excited at 356 nm and the emission scans were recorded from 370–500 nm. [Poly(dA)•2poly(dT)] = 15 µM/bt. [naphthalene diimide] = 0.5 µm to 15µm. [T] = 10 °C. n = base triplet/drug. Buffer conditions: sodium cacodylate (10 mM), EDTA (0.5 mM), KCl (150 mM), pH (5.5).