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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Biochimie. 2013 Jan 16;95(6):1177–1184. doi: 10.1016/j.biochi.2013.01.005

Figure 5.

Figure 5

HGF suppresses TGFβ-mediated ROS generation and its cytotoxic effect. (A) ROS generation by TGFβ treatment. Cells were pretreated with 50 ng/ml HGF for 12 h and then exposed to 5 ng/ml TGFβ for 0.5, 1, 3, 6, and, 12 h, de-esterified DCFH fluorescence was recorded after 30 min incubation with DCFH (5 μM). Data are expressed as fold change relative to the untreated cells. Data represents the means ± SEM of at least three independent experiments carried out in triplicate. * P ≤ 0.05 vs untreated cells (0 h); # P ≤ 0.05 vs 1 h treatment with TGFβ, & P ≤ 0.05 vs 3 h treatment with TGFβ. (B) Cell viability in cultures pretreated with HGF for 12 h and then exposed to 5 ng/ml TGFβ for 24 h. Data represent the mean ± SEM of at least three independent experiments. * P ≤ 0.05 vs untreated cells (NT); # P ≤ vs TGFβ treated cells.