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. 1993 Dec;13(12):7487–7495. doi: 10.1128/mcb.13.12.7487

Yin-yang 1 activates the c-myc promoter.

K J Riggs 1, S Saleque 1, K K Wong 1, K T Merrell 1, J S Lee 1, Y Shi 1, K Calame 1
PMCID: PMC364820  PMID: 8246966

Abstract

Previous studies on the murine c-myc promoter demonstrated that a ubiquitously present protein, common factor 1 (CF1), bound at two sites located -260 and -390 bp from the P1 transcription start site. CF1 has been purified to near homogeneity and shown to be identical to the zinc finger protein Yin-yang 1 (YY1) as judged by similarity of molecular weight and other biochemical properties, immunological cross-reactivity, and the ability of recombinant YY1 to bind to CF1 sites. In cotransfection experiments, YY1 is a strong activator of transcription from c-myc promoter-based reporters. Furthermore, in murine erythroleukemia cells, overexpressed YY1 causes increased levels of c-myc mRNA initiated from both major transcription initiation sites of the endogenous c-myc gene.

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