Table 1.
(A) Spectral Overlap between Donors and Acceptors,a (B) Quantum Yields of Donor Probes Bound to the Noted Receptor Constructs, and (C) Efficiency of Energy Transfer between Donors and Acceptorsa
| site of receptor labeling | J × 10–15 cm3 M–1 |
||
|---|---|---|---|
| Aladan24-CCK | Aladan29-CCK | Aladan33-CCK | |
| (A) Spectral Overlap between Donors and Acceptors | |||
| C94 | 3.6 ± 1.0 | 4.2 ± 0.8 | 3.5 ± 1.2 |
| N102C | 0.9 ± 0.3 | 0.9 ± 0.1 | 1.2 ± 0.3 |
| A204C | 8.3 ± 3.9 | 3.3 ± 1.4 | 10.0 ± 3.1 |
| T341C | 0.7 ± 0.3 | 0.9 ± 0.4 | 0.6 ± 0.2 |
| site of receptor labeling | Aladan24-CCK | Aladan29-CCK | Aladan33-CCK |
|---|---|---|---|
| (B) Quantum Yields of Donor Probes Bound to the Noted Receptor Constructs | |||
| C94 | 0.29 | 0.29 | 0.33 |
| N102C | 0.29 | 0.30 | 0.33 |
| A204C | 0.30 | 0.27 | 0.31 |
| T341C | 0.30 | 0.28 | 0.32 |
| site of receptor labeling | % |
||
|---|---|---|---|
| Aladan24-CCK | Aladan29-CCK | Aladan33-CCK | |
| (C) Efficiency of Energy Transfer between Donors and Acceptors | |||
| C94 | 56 ± 1.4 | 55 ± 1.1 | 58 ± 1.7 |
| N102C | 64 ± 2.3 | 66 ± 1.1 | 70 ± 3.5 |
| A204C | 53 ± 1.7 | 54 ± 2.3 | 53 ± 1.8 |
| T341C | 64 ± 1.8 | 67 ± 1.2 | 66 ± 1.5 |
a
Values are expressed as means ± S.E.M. of data from a minimum of four to five independent experiments.