Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1987 Jan;7(1):532–534. doi: 10.1128/mcb.7.1.532

Cell cycle-dependent effects on deoxyribonucleotide and DNA labeling by nucleoside precursors in mammalian cells.

J M Leeds, C K Mathews
PMCID: PMC365098  PMID: 3561401

Abstract

dCTP pools equilibrated to equivalent specific activities in Chinese hamster ovary cells or in nuclei after incubation of cells with radiolabeled nucleosides, indicating that dCTP in nuclei does not constitute a distinct metabolic pool. In the G1 phase, [5-3H]deoxycytidine labeled dCTP to unexpectedly high specific activities. This may explain reports of replication-excluded DNA precursor pools.

Full text

PDF
532

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Engström Y., Rozell B., Hansson H. A., Stemme S., Thelander L. Localization of ribonucleotide reductase in mammalian cells. EMBO J. 1984 Apr;3(4):863–867. doi: 10.1002/j.1460-2075.1984.tb01897.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fridland A. DNA precursors in eukaryotic cells. Nat New Biol. 1973 May 23;243(125):105–107. [PubMed] [Google Scholar]
  3. Hellgren D., Nilsson S., Reichard P. Effects of arabinosyl-cytosine on thymidine triphosphate pools and polyoma DNA replication. Biochem Biophys Res Commun. 1979 May 14;88(1):16–22. doi: 10.1016/0006-291x(79)91690-5. [DOI] [PubMed] [Google Scholar]
  4. Hordern J., Henderson J. F. Comparison of purine and pyrimidine metabolism in G1 and S phases of HeLa and Chinese hamster ovary cells. Can J Biochem. 1982 Apr;60(4):422–433. doi: 10.1139/o82-050. [DOI] [PubMed] [Google Scholar]
  5. Hunting D., Henderson J. F. Determination of deoxyribonucleoside triphosphates using DNA polymerase: a critical evaluation. Can J Biochem. 1981 Sep;59(9):723–727. doi: 10.1139/o81-100. [DOI] [PubMed] [Google Scholar]
  6. Kuebbing D., Werner R. A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3333–3336. doi: 10.1073/pnas.72.9.3333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leeds J. M., Slabaugh M. B., Mathews C. K. DNA precursor pools and ribonucleotide reductase activity: distribution between the nucleus and cytoplasm of mammalian cells. Mol Cell Biol. 1985 Dec;5(12):3443–3450. doi: 10.1128/mcb.5.12.3443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mathews C. K. Enzymatic channeling of DNA precursors. Basic Life Sci. 1985;31:47–66. doi: 10.1007/978-1-4613-2449-2_4. [DOI] [PubMed] [Google Scholar]
  9. Mathews C. K., Slabaugh M. B. Eukaryotic DNA metabolism. Are deoxyribonucleotides channeled to replication sites? Exp Cell Res. 1986 Feb;162(2):285–295. doi: 10.1016/0014-4827(86)90335-6. [DOI] [PubMed] [Google Scholar]
  10. Maybaum J., Cohen M. B., Sadee W. In vivo rates of pyrimidine nucleotide metabolism in intact mouse T-lymphoma (s-49) cells treated with 5-fluorouracil. J Biol Chem. 1981 Mar 10;256(5):2126–2130. [PubMed] [Google Scholar]
  11. McCormick P. J., Danhauser L. L., Rustum Y. M., Bertram J. S. Changes in ribo- and deoxyribonucleoside triphosphate pools within the cell cycle of a synchronized mouse fibroblast cell line. Biochim Biophys Acta. 1983 Mar 15;756(1):36–40. doi: 10.1016/0304-4165(83)90021-1. [DOI] [PubMed] [Google Scholar]
  12. Moyer J. D., Henderson J. F. Compartmentation of intracellular nucleotides in mammalian cells. CRC Crit Rev Biochem. 1985;19(1):45–61. doi: 10.3109/10409238509086787. [DOI] [PubMed] [Google Scholar]
  13. Nguyen B. T., Sadée W. Compartmentation of guanine nucleotide precursors for DNA synthesis. Biochem J. 1986 Mar 1;234(2):263–269. doi: 10.1042/bj2340263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nicander B., Reichard P. Dynamics of pyrimidine deoxynucleoside triphosphate pools in relationship to DNA synthesis in 3T6 mouse fibroblasts. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1347–1351. doi: 10.1073/pnas.80.5.1347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nicander B., Reichard P. Evidence for the involvement of substrate cycles in the regulation of deoxyribonucleoside triphosphate pools in 3T6 cells. J Biol Chem. 1985 Aug 5;260(16):9216–9222. [PubMed] [Google Scholar]
  16. North T. W., Bestwick R. K., Mathews C. K. Detection of activities that interfere with the enzymatic assay of deoxyribonucleoside 5'-triphosphates. J Biol Chem. 1980 Jul 25;255(14):6640–6645. [PubMed] [Google Scholar]
  17. Scott F. W., Forsdyke D. R. Isotope-dilution analysis of the effects of deoxyguanosine and deoxyadenosine on the incorporation of thymidine and deoxycytidine by hydroxyurea-treated thymus cells. Biochem J. 1980 Sep 15;190(3):721–730. doi: 10.1042/bj1900721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Snyder R. D. The role of deoxynucleoside triphosphate pools in the inhibition of DNA-excision repair and replication in human cells by hydroxyurea. Mutat Res. 1984 Mar-Apr;131(3-4):163–172. doi: 10.1016/0167-8817(84)90057-9. [DOI] [PubMed] [Google Scholar]
  19. Wickremasinghe R. G., Hoffbrand A. V. Reduced rate of DNA replication fork movement in megaloblastic anemia. J Clin Invest. 1980 Jan;65(1):26–36. doi: 10.1172/JCI109656. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES