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. 1987 Jan;7(1):545–548. doi: 10.1128/mcb.7.1.545

Identification of a promoter element located upstream from the hepatitis B virus X gene.

M Treinin, O Laub
PMCID: PMC365101  PMID: 3494193

Abstract

We have analyzed a series of plasmids in which the sequences located upstream from the hepatitis B virus (HBV) X gene were linked to the chloramphenicol acetyl transferase (CAT) gene. Expression of the marker CAT gene in transfected cells clearly demonstrated that sequences preceding the X gene contain an active promoter. RNA mapping by primer extension indicated that the RNA encoded by the X gene promoter initiates at multiple sites spanning nucleotides 1250 to 1350 on the HBV genome. Deletion within the adjacent HBV enhancer element region significantly reduced the activity of the X gene promoter, suggesting that the X gene promoter requires the enhancer element for maximal activity.

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Selected References

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