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. 1987 Jan;7(1):549–551. doi: 10.1128/mcb.7.1.549

A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.

G Akusjärvi, C Svensson, O Nygård
PMCID: PMC365102  PMID: 2436038

Abstract

The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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