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. 1987 Apr;7(4):1535–1540. doi: 10.1128/mcb.7.4.1535

Differential expression of the ras gene family in mice.

J Leon, I Guerrero, A Pellicer
PMCID: PMC365242  PMID: 3600635

Abstract

We compared the expression of the ras gene family (H-ras, K-ras, and N-ras) in adult mouse tissues and during development. We found substantial variations in expression among different organs and in the amounts of the different transcripts originating from each gene, especially for the N-ras gene. The expression patterns were consistent with the reported preferential tissue activation of ras genes and suggested different cellular functions for each of the ras genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amara S. G., Evans R. M., Rosenfeld M. G. Calcitonin/calcitonin gene-related peptide transcription unit: tissue-specific expression involves selective use of alternative polyadenylation sites. Mol Cell Biol. 1984 Oct;4(10):2151–2160. doi: 10.1128/mcb.4.10.2151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balmain A., Ramsden M., Bowden G. T., Smith J. Activation of the mouse cellular Harvey-ras gene in chemically induced benign skin papillomas. Nature. 1984 Feb 16;307(5952):658–660. doi: 10.1038/307658a0. [DOI] [PubMed] [Google Scholar]
  3. Bar-Sagi D., Feramisco J. R. Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. Cell. 1985 Oct;42(3):841–848. doi: 10.1016/0092-8674(85)90280-6. [DOI] [PubMed] [Google Scholar]
  4. Battistuzzi G., D'Urso M., Toniolo D., Persico G. M., Luzzatto L. Tissue-specific levels of human glucose-6-phosphate dehydrogenase correlate with methylation of specific sites at the 3' end of the gene. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1465–1469. doi: 10.1073/pnas.82.5.1465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bellard M., Dretzen G., Bellard F., Kaye J. S., Pratt-Kaye S., Chambon P. Hormonally induced alterations of chromatin structure in the polyadenylation and transcription termination regions of the chicken ovalbumin gene. EMBO J. 1986 Mar;5(3):567–574. doi: 10.1002/j.1460-2075.1986.tb04248.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Birnstiel M. L., Busslinger M., Strub K. Transcription termination and 3' processing: the end is in site! Cell. 1985 Jun;41(2):349–359. doi: 10.1016/s0092-8674(85)80007-6. [DOI] [PubMed] [Google Scholar]
  7. Bizub D., Wood A. W., Skalka A. M. Mutagenesis of the Ha-ras oncogene in mouse skin tumors induced by polycyclic aromatic hydrocarbons. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6048–6052. doi: 10.1073/pnas.83.16.6048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Capetanaki Y. G., Ngai J., Flytzanis C. N., Lazarides E. Tissue-specific expression of two mRNA species transcribed from a single vimentin gene. Cell. 1983 Dec;35(2 Pt 1):411–420. doi: 10.1016/0092-8674(83)90174-5. [DOI] [PubMed] [Google Scholar]
  9. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  10. DeFeo-Jones D., Scolnick E. M., Koller R., Dhar R. ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae. Nature. 1983 Dec 15;306(5944):707–709. doi: 10.1038/306707a0. [DOI] [PubMed] [Google Scholar]
  11. DeFeo D., Gonda M. A., Young H. A., Chang E. H., Lowy D. R., Scolnick E. M., Ellis R. W. Analysis of two divergent rat genomic clones homologous to the transforming gene of Harvey murine sarcoma virus. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3328–3332. doi: 10.1073/pnas.78.6.3328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ellis R. W., DeFeo D., Maryak J. M., Young H. A., Shih T. Y., Chang E. H., Lowy D. R., Scolnick E. M. Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virus. J Virol. 1980 Nov;36(2):408–420. doi: 10.1128/jvi.36.2.408-420.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ellis R. W., Defeo D., Shih T. Y., Gonda M. A., Young H. A., Tsuchida N., Lowy D. R., Scolnick E. M. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes. Nature. 1981 Aug 6;292(5823):506–511. doi: 10.1038/292506a0. [DOI] [PubMed] [Google Scholar]
  14. George D. L., Scott A. F., Trusko S., Glick B., Ford E., Dorney D. J. Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells. EMBO J. 1985 May;4(5):1199–1203. doi: 10.1002/j.1460-2075.1985.tb03760.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Guerrero I., Calzada P., Mayer A., Pellicer A. A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene. Proc Natl Acad Sci U S A. 1984 Jan;81(1):202–205. doi: 10.1073/pnas.81.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Guerrero I., Villasante A., Corces V., Pellicer A. Loss of the normal N-ras allele in a mouse thymic lymphoma induced by a chemical carcinogen. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7810–7814. doi: 10.1073/pnas.82.23.7810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Guerrero I., Villasante A., D'Eustachio P., Pellicer A. Isolation, characterization, and chromosome assignment of mouse N-ras gene from carcinogen-induced thymic lymphoma. Science. 1984 Sep 7;225(4666):1041–1043. doi: 10.1126/science.6089339. [DOI] [PubMed] [Google Scholar]
  18. Guerrero I., Villasante A., Diamond L., Berman J. W., Newcomb E. W., Steinberg J. J., Lake R., Pellicer A. Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea. Leuk Res. 1986;10(7):851–858. doi: 10.1016/0145-2126(86)90306-1. [DOI] [PubMed] [Google Scholar]
  19. Guerrero I., Wong H., Pellicer A., Burstein D. E. Activated N-ras gene induces neuronal differentiation of PC12 rat pheochromocytoma cells. J Cell Physiol. 1986 Oct;129(1):71–76. doi: 10.1002/jcp.1041290111. [DOI] [PubMed] [Google Scholar]
  20. Hankins W. D., Scolnick E. M. Harvey and Kirsten sarcoma viruses promote the growth and differentiation of erythroid precursor cells in vitro. Cell. 1981 Oct;26(1 Pt 1):91–97. doi: 10.1016/0092-8674(81)90036-2. [DOI] [PubMed] [Google Scholar]
  21. Helfman D. M., Cheley S., Kuismanen E., Finn L. A., Yamawaki-Kataoka Y. Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation. Mol Cell Biol. 1986 Nov;6(11):3582–3595. doi: 10.1128/mcb.6.11.3582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lumpkin C. K., Knepper J. E., Butel J. S., Smith J. R., Pereira-Smith O. M. Mitogenic effects of the proto-oncogene and oncogene forms of c-H-ras DNA in human diploid fibroblasts. Mol Cell Biol. 1986 Aug;6(8):2990–2993. doi: 10.1128/mcb.6.8.2990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Meijlink F., Curran T., Miller A. D., Verma I. M. Removal of a 67-base-pair sequence in the noncoding region of protooncogene fos converts it to a transforming gene. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4987–4991. doi: 10.1073/pnas.82.15.4987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Milcarek C., Hall B. Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites. Mol Cell Biol. 1985 Oct;5(10):2514–2520. doi: 10.1128/mcb.5.10.2514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mulcahy L. S., Smith M. R., Stacey D. W. Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells. Nature. 1985 Jan 17;313(5999):241–243. doi: 10.1038/313241a0. [DOI] [PubMed] [Google Scholar]
  26. Müller R., Slamon D. J., Adamson E. D., Tremblay J. M., Müller D., Cline M. J., Verma I. M. Transcription of c-onc genes c-rasKi and c-fms during mouse development. Mol Cell Biol. 1983 Jun;3(6):1062–1069. doi: 10.1128/mcb.3.6.1062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Müller R., Slamon D. J., Tremblay J. M., Cline M. J., Verma I. M. Differential expression of cellular oncogenes during pre- and postnatal development of the mouse. Nature. 1982 Oct 14;299(5884):640–644. doi: 10.1038/299640a0. [DOI] [PubMed] [Google Scholar]
  28. Neuman-Silberberg F. S., Schejter E., Hoffmann F. M., Shilo B. Z. The Drosophila ras oncogenes: structure and nucleotide sequence. Cell. 1984 Jul;37(3):1027–1033. doi: 10.1016/0092-8674(84)90437-9. [DOI] [PubMed] [Google Scholar]
  29. Noda M., Ko M., Ogura A., Liu D. G., Amano T., Takano T., Ikawa Y. Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. Nature. 1985 Nov 7;318(6041):73–75. doi: 10.1038/318073a0. [DOI] [PubMed] [Google Scholar]
  30. Pfeifer-Ohlsson S., Goustin A. S., Rydnert J., Wahlström T., Bjersing L., Stehelin D., Ohlsson R. Spatial and temporal pattern of cellular myc oncogene expression in developing human placenta: implications for embryonic cell proliferation. Cell. 1984 Sep;38(2):585–596. doi: 10.1016/0092-8674(84)90513-0. [DOI] [PubMed] [Google Scholar]
  31. Ponzetto C., Wolgemuth D. J. Haploid expression of a unique c-abl transcript in the mouse male germ line. Mol Cell Biol. 1985 Jul;5(7):1791–1794. doi: 10.1128/mcb.5.7.1791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Quintanilla M., Brown K., Ramsden M., Balmain A. Carcinogen-specific mutation and amplification of Ha-ras during mouse skin carcinogenesis. Nature. 1986 Jul 3;322(6074):78–80. doi: 10.1038/322078a0. [DOI] [PubMed] [Google Scholar]
  33. Reymond C. D., Gomer R. H., Mehdy M. C., Firtel R. A. Developmental regulation of a Dictyostelium gene encoding a protein homologous to mammalian ras protein. Cell. 1984 Nov;39(1):141–148. doi: 10.1016/0092-8674(84)90199-5. [DOI] [PubMed] [Google Scholar]
  34. Segal D., Shilo B. Z. Tissue localization of Drosophila melanogaster ras transcripts during development. Mol Cell Biol. 1986 Jun;6(6):2241–2248. doi: 10.1128/mcb.6.6.2241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Setzer D. R., McGrogan M., Nunberg J. H., Schimke R. T. Size heterogeneity in the 3' end of dihydrofolate reductase messenger RNAs in mouse cells. Cell. 1980 Nov;22(2 Pt 2):361–370. doi: 10.1016/0092-8674(80)90346-3. [DOI] [PubMed] [Google Scholar]
  36. Shimizu K., Goldfarb M., Perucho M., Wigler M. Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line. Proc Natl Acad Sci U S A. 1983 Jan;80(2):383–387. doi: 10.1073/pnas.80.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Simcox A. A., Cheney C. M., Hoffman E. P., Shearn A. A deletion of the 3' end of the Drosophila melanogaster hsp70 gene increases stability of mutant mRNA during recovery from heat shock. Mol Cell Biol. 1985 Dec;5(12):3397–3402. doi: 10.1128/mcb.5.12.3397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Slamon D. J., Cline M. J. Expression of cellular oncogenes during embryonic and fetal development of the mouse. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7141–7145. doi: 10.1073/pnas.81.22.7141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Swanson M. E., Elste A. M., Greenberg S. M., Schwartz J. H., Aldrich T. H., Furth M. E. Abundant expression of ras proteins in Aplysia neurons. J Cell Biol. 1986 Aug;103(2):485–492. doi: 10.1083/jcb.103.2.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Tanaka T., Ida N., Shimoda H., Waki C., Slamon D. J., Cline M. J. Organ specific expression of ras oncoproteins during growth and development of the rat. Mol Cell Biochem. 1986 Apr;70(1):97–104. doi: 10.1007/BF00233807. [DOI] [PubMed] [Google Scholar]
  41. Varmus H. E. The molecular genetics of cellular oncogenes. Annu Rev Genet. 1984;18:553–612. doi: 10.1146/annurev.ge.18.120184.003005. [DOI] [PubMed] [Google Scholar]
  42. Waneck G. L., Keyes L., Rosenberg N. Abelson virus drives the differentiation of Harvey virus-infected erythroid cells. Cell. 1986 Jan 31;44(2):337–344. doi: 10.1016/0092-8674(86)90768-3. [DOI] [PubMed] [Google Scholar]

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