Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1987 May;7(5):2031–2034. doi: 10.1128/mcb.7.5.2031

Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.

D E Brash, R R Reddel, M Quanrud, K Yang, M P Farrell, C C Harris
PMCID: PMC365315  PMID: 3037341

Abstract

Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).

Full text

PDF
2031

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COLTER J. S., BIRD H. H., MOYER A. W., BROWN R. A. Infectivity of ribonucleic acid isolated from virus-infected tissues. Virology. 1957 Dec;4(3):522–532. doi: 10.1016/0042-6822(57)90084-3. [DOI] [PubMed] [Google Scholar]
  2. Cepko C. L., Roberts B. E., Mulligan R. C. Construction and applications of a highly transmissible murine retrovirus shuttle vector. Cell. 1984 Jul;37(3):1053–1062. doi: 10.1016/0092-8674(84)90440-9. [DOI] [PubMed] [Google Scholar]
  3. Dimayorca G. A., Eddy B. E., Stewart S. E., Hunter W. S., Friend C., Bendich A. ISOLATION OF INFECTIOUS DEOXYRIBONUCLEIC ACID FROM SE POLYOMA-INFECTED TISSUE CULTURES. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1805–1808. doi: 10.1073/pnas.45.12.1805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GIERER A., SCHRAMM G. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature. 1956 Apr 14;177(4511):702–703. doi: 10.1038/177702a0. [DOI] [PubMed] [Google Scholar]
  5. GRAHAM A. F., SIMINOVITCH L. Significance of ribonucleic acid and deoxyribonucleic acid turnover studies. J Histochem Cytochem. 1956 Nov;4(6):508–515. doi: 10.1177/4.6.508. [DOI] [PubMed] [Google Scholar]
  6. Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorman C., Padmanabhan R., Howard B. H. High efficiency DNA-mediated transformation of primate cells. Science. 1983 Aug 5;221(4610):551–553. doi: 10.1126/science.6306768. [DOI] [PubMed] [Google Scholar]
  8. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  9. HOLLAND J. J., HOYER B. H., McLAREN L. C., SYVERTON J. T. Enteroviral ribonucleic acid. I. Recovery from virus and assimilation by cells. J Exp Med. 1960 Nov 1;112:821–839. doi: 10.1084/jem.112.5.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harris C. C. Human tissues and cells in carcinogenesis research. Cancer Res. 1987 Jan 1;47(1):1–10. [PubMed] [Google Scholar]
  11. Hennings H., Michael D., Cheng C., Steinert P., Holbrook K., Yuspa S. H. Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell. 1980 Jan;19(1):245–254. doi: 10.1016/0092-8674(80)90406-7. [DOI] [PubMed] [Google Scholar]
  12. ITO Y. A tumor-producing factor extracted by phenol from papillomatous tissue (Shope) of cottontail rabbits. Virology. 1960 Dec;12:596–601. doi: 10.1016/0042-6822(60)90182-3. [DOI] [PubMed] [Google Scholar]
  13. Kawai S., Nishizawa M. New procedure for DNA transfection with polycation and dimethyl sulfoxide. Mol Cell Biol. 1984 Jun;4(6):1172–1174. doi: 10.1128/mcb.4.6.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mueller C., Graessmann A., Graessmann M. Mapping of early SV40-specific functions by microinjection of different early viral DNA fragments. Cell. 1978 Oct;15(2):579–585. doi: 10.1016/0092-8674(78)90026-0. [DOI] [PubMed] [Google Scholar]
  15. Neumann E., Schaefer-Ridder M., Wang Y., Hofschneider P. H. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1982;1(7):841–845. doi: 10.1002/j.1460-2075.1982.tb01257.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pagano J. S., McCutchan J. H., Vaheri A. Factors influencing the enhancement of the infectivity of poliovirus ribonucleic acid by diethylaminoethyl-dextran. J Virol. 1967 Oct;1(5):891–897. doi: 10.1128/jvi.1.5.891-897.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pagano J. S., Vaheri A. Enhancement of infectivity of poliovirus RNA with diethylaminoethyl-dextran (DEAE-D). Arch Gesamte Virusforsch. 1965;17(3):456–464. doi: 10.1007/BF01241201. [DOI] [PubMed] [Google Scholar]
  18. Peehl D. M., Ham R. G. Clonal growth of human keratinocytes with small amounts of dialyzed serum. In Vitro. 1980 Jun;16(6):526–540. doi: 10.1007/BF02626466. [DOI] [PubMed] [Google Scholar]
  19. Queen C., Baltimore D. Immunoglobulin gene transcription is activated by downstream sequence elements. Cell. 1983 Jul;33(3):741–748. doi: 10.1016/0092-8674(83)90016-8. [DOI] [PubMed] [Google Scholar]
  20. Schaefer-Ridder M., Wang Y., Hofschneider P. H. Liposomes as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene. Science. 1982 Jan 8;215(4529):166–168. doi: 10.1126/science.7053567. [DOI] [PubMed] [Google Scholar]
  21. Schaffner W. Direct transfer of cloned genes from bacteria to mammalian cells. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2163–2167. doi: 10.1073/pnas.77.4.2163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Southern P. J., Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet. 1982;1(4):327–341. [PubMed] [Google Scholar]
  23. Vaheri A., Pagano J. S. Infectious poliovirus RNA: a sensitive method of assay. Virology. 1965 Nov;27(3):434–436. doi: 10.1016/0042-6822(65)90126-1. [DOI] [PubMed] [Google Scholar]
  24. WEIL R. A quantitative assay for a subviral infective agent related to polyoma virus. Virology. 1961 May;14:46–53. doi: 10.1016/0042-6822(61)90130-1. [DOI] [PubMed] [Google Scholar]
  25. Wigler M., Pellicer A., Silverstein S., Axel R., Urlaub G., Chasin L. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373–1376. doi: 10.1073/pnas.76.3.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoakum G. H., Korba B. E., Lechner J. F., Tokiwa T., Gazdar A. F., Seeley T., Siegel M., Leeman L., Autrup H., Harris C. C. High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells. Science. 1983 Oct 28;222(4622):385–389. doi: 10.1126/science.6194563. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES