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. 1987 Jun;7(6):2087–2096. doi: 10.1128/mcb.7.6.2087

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.

B Sauer
PMCID: PMC365329  PMID: 3037344

Abstract

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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