Table 1.
—Experimental Design and Numbers of Animals per Group
| Variable | EBD, Lung Water (8 h) | MIP-2, TGF-β1, MPO (8 h) | Lumican and ERK1/2 Protein, Lumican mRNA (2 h) | Collagen, MDA (8 h) | IF, Trichrome Stain, EM (8 h) |
| Control, wild type | 5 | 5 | 5 | 5 | 5 |
| Control, lum−/− | 5 | 5 | 5 | 5 | 5 |
| Vt 6 mL/kg, wild type | 5 | 5 | 5 | 5 | 5 |
| Vt 10 mL/kg, wild type | 4 | 4 | 4 | 0 | 0 |
| Vt 30 mL/kg, wild type | 5 | 5 | 5 | 5 | 5 |
| Vt 30 mL/kg, wild type with PD98059 | 5 | 5 | 5 | 5 | 5 |
| Vt 30 mL/kg, lum−/− | 5 | 5 | 5 | 5 | 5 |
Control = spontaneously breathing, nonventilated mice; EBD = Evans blue dye; EM = electron microscopy; ERK = extracellular signal-regulated kinase; IF = immunofluorescence; lum2212/2212 = lumican null; MDA = malondialdehyde; MIP-2 = macrophage-inflammatory protein-2; MPO = myeloperoxidase; TGF-β1 = transforming growth factor-β1; Vt = tidal volume.