Table 1.
List of proteins identified on intact E. faecalis V583 biofilms by top down and bottom up proteomic approaches.
| Top Down | |||||
|---|---|---|---|---|---|
| Database | Sequence Reference | Protein | MASCOT score(a) | Sequence Coverage (%) | Theoretical mol. wt. |
| V583 | gi/29376414 | EF1885 | 65 | 100 | 4637 |
| V583 | gi/29376284 | EF1734 | 104 | 100 | 8165 |
| Bottom Up | ||||||
|---|---|---|---|---|---|---|
| Database | Sequence Reference | Protein | MASCOT score(b) | Peptide m/z used for protein identification | Theoritical mol. wt of the identified protein | Protein subcellular localization |
| V583 | gi|29343947 | enolase | 68 | 717.4, 1188.6, 1851.9 | 46482 | Cytoplasm |
| V583 | gi|29343950 | glyceraldehyde 3-phosphate dehydrogenases | 47 | 749.4, 819.3 | 35749 | Cytoplasm |
| V583 | gi|29342693 | tyrosyl-tRNA synthetase | 37 | 1389.4, 1487.4 | 47231 | Cytoplasm |
| V583 | gi|29344777 | hypothetical protein EF_2843 | 36 | 828.5 | 8359 | Cytoplasm |
| V583 | gi|29344997 | BioY family protein | 34 | 730.1 | 19446 | Membrane |
| V583 | gi|29342815 | excinuclease ABC, subunit B | 34 | 1701.8 | 76054 | Cytoplasm |
| V583 | gi|29344009 | hypothetical protein EF_2033 | 34 | 788.4 | 4498 | Cytoplasm |
| V583 | gi|29342305 | translation elongation factor Tu | 32 | 1139.6, 1702.9, 1862.9, 2184.0 | 43361 | Cytoplasm |
| V583 | gi|29344612 | helicase, putative, RecD/TraA family | 32 | 1863 | 97416 | Cytoplasm |
| V583 | gi|29344600 | spermidine/putrescine ABC transporter, permease protein | 29 | 629.4 | 31484 | Membrane |
| V583 | gi|29344133 | GTP-binding protein | 28 | 712.1 | 46992 | Cytoplasm |
(a)
Protein score greater than 51 are significant (p<0.05)
(b)
Protein score greater than 26 indicate identity or extensive homology (p<0.05)