Figure 4. Induction of MART-1 specific CD8⁺ T cells by CD14⁺ and CD14⁻ dendritic cells matured in the presence of interleukin-10. (A–C) Autologous HLA-A2⁺ monocyte-derived dendritic cells (MoDCs) matured in the presence (IL-10-DCs) or in the absence (mDCs) of interleukin-10 (IL-10) and sorted by CD14 expression were loaded with MART-1_26–35L and co-cultured with MART-1-specific CD8β⁺ T cells in multiple (n = 6) parallel cultures per condition per donor. (A) Representative examples of MART-1_26–35L HLA-A2 tetramer binding by CD8⁺ T cells after a single 10-d in vitro stimulation for different mDC cultures. (B) Mean 10-d priming efficiencies (in % HLA-A2⁺ tetramer-bound T cells) from 5 healthy donors, *p < 0.05. (C) Tetramer binding levels (by mean fluorescence intensity) from tetramer-positive priming cultures, *p < 0.05.