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. 1988 Oct;8(10):4561–4565. doi: 10.1128/mcb.8.10.4561

Amplified gene for the epidermal growth factor receptor in a human glioblastoma cell line encodes an enzymatically inactive protein.

A Wells 1, J M Bishop 1, D Helmeste 1
PMCID: PMC365536  PMID: 3263568

Abstract

The gene encoding the receptor for epidermal growth factor was amplified two- to fivefold in the human glioblastoma cell line SF268. The amplified gene gave rise to abundant quantities of receptor that bound EGF with a high affinity (Kd, 0.35 nM). The binding of ligand failed to elicit cellular DNA synthesis, however, and the receptor was enzymatically inactive. We presume that the amplified receptor gene carries a mutation(s) that affects several aspects of the receptor's function. Characterization of the mutation(s) may illuminate how structure dictates function in the receptor protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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