Skip to main content
. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Neuropsychopharmacology. 2009 Jun 17;34(10):2344–2357. doi: 10.1038/npp.2009.64

Figure 2. The neurotrophic effect of memanatine was astroglia-dependent.

Figure 2

(A) Memantine (10 µM) or vehicle was added to the following different cell cultures: neuron-glia cultures, neuron-enriched cultures, neuron-microglia co-cultures by adding 7.5 × 104/well of enriched microglia to the neuron-enriched cultures, and microglia-depleted cultures. DA neuronal function was quantified by the [3H] DA uptake assay. Results were expressed as a percentage of corresponding control cultures and were the mean ± S.E.M. from three independent experiments in triplicate. ***, p< 0.001 compared with corresponding control cultures. (B, C) The astrogliaconditioned media was prepared from astroglia-enriched cultures treated for 48 h with memantine (10 µM) (ACM-MMT) or vehicle (ACM). Seven days after adding the conditioned media to the neuron-enriched cultures, DA neuronal function was quantified by the [3H] DA uptake assay (B) and the cultures were stained with TH antibody (C). Results were expressed as a percentage of the vehicle-treated non-conditioned control cultures and were the mean ± S.E.M. from three independent experiments in triplicate. **, p<0.01, ***, p<0.001 compared with the control cultures; #, p<0.05 compared with the ACM-treated cultures.