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. 1988 Nov;8(11):5035–5039. doi: 10.1128/mcb.8.11.5035

Expression and processing of a recombinant human macrophage colony-stimulating factor in mouse cells.

M M Manos 1
PMCID: PMC365597  PMID: 3264878

Abstract

A human macrophage colony-stimulating factor encoded by a 4-kilobase cDNA was expressed with bovine papillomavirus vectors in mouse cells. Pulse-chase analyses revealed that the 62-kilodalton (kDa) translation product was glycosylated, cleaved, and efficiently secreted within 1 h of synthesis. The secreted product contained both N-linked and O-linked oligosaccharide chains. Macrophage colony-stimulating factor was present extracellularly as an 80-kDa homodimer and as a multimeric species of greater than 200 kDa that may be associated with other glycoproteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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