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. 1988 Dec;8(12):5432–5438. doi: 10.1128/mcb.8.12.5432

Prolactin upstream factor I mediates cell-specific transcription.

Z D Cao 1, E A Barron 1, Z D Sharp 1
PMCID: PMC365646  PMID: 3244361

Abstract

DNA sequence-specific chromatography was used to purify prolactin upstream factor I (PUF-I) approximately 10,000- to 20,000-fold from rat GH3 cells. The purified transcription factor reconstituted enhanced pituitary-specific prolactin RNA synthesis in nonpituitary in vitro transcription assays. In vitro mutagenesis demonstrated that the capacity to stimulate prolactin gene transcription was directly correlated with PUF-I binding to an A+T-rich region located from -63 to -36 in the prolactin 5'-flanking DNA. We propose that PUF-I is a critical modulator of transcriptional activity in pituitary cells and has a central role in the stimulation of prolactin gene transcription in the mammalian pituitary lactotroph.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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