Abstract
Operational and constructional details are given of an apparatus which permits simultaneous enzyme extraction from up to 16 cell samples under standardized conditions. The main advantages of the method are: (a) individual samples remain in the same container throughout the whole operation; (b) the samples can be stirred continuously and uniformly during extraction; (c) rapid changes of extractant are facilitated by a simple draining operation; (d) the extraction temperature can be strictly controlled. The method is rapid, efficient, and reproducible in extracting a number of enzymes from cultured rose cells. Other uses of the apparatus are discussed, particularly in relation to the extraction of protein, nucleic acids, and some low molecular weight materials.
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- Davies M. E. Acetolactate and Acetoin Synthesis in Ripening Peas. Plant Physiol. 1964 Jan;39(1):53–59. doi: 10.1104/pp.39.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kivilaan A., Beaman T. C., Bandurski R. S. Enzymatic activities associated with cell wall preparations from corn coleoptiles. Plant Physiol. 1961 Sep;36(5):605–610. doi: 10.1104/pp.36.5.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
- Woolfolk C. A., Shapiro B., Stadtman E. R. Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli. Arch Biochem Biophys. 1966 Sep 26;116(1):177–192. doi: 10.1016/0003-9861(66)90026-9. [DOI] [PubMed] [Google Scholar]