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. 1971 Jan;47(1):91–97. doi: 10.1104/pp.47.1.91

Synergistic Effects of Metabolically Related Amino Acids on the Growth of a Multicellular Plant

II. Studies of 14C-Amino Acid Incorporation 1,2

Valgene L Dunham a,3, J K Bryan a
PMCID: PMC365818  PMID: 5543785

Abstract

The synergistic inhibition of the growth of Marchantia polymorpha gemmalings by lysine and threonine and its prevention by methionine has been investigated utilizing 14C-labeled amino acids. Experiments involving the uptake of 14C-lysine or 14C-threonine in the presence or absence of methionine indicated that the synergistic growth effects were not a result of altered amino acid uptake. These data, as well as direct chemical analysis, indicated that growth inhibition was correlated with an inhibition of protein synthesis. Experiments utilizing 14C-aspartic acid revealed that the presence of lysine and threonine resulted in increased 14CO2 production and an accumulation of soluble 14C-aspartic acid and labeled ninhydrin-positive compounds. These metabolic alterations were prevented when methionine was also included in the growth media. A model depicting a sequence of events which involve the interaction of regulatory mechanisms is suggested to account for the effects of specific amino acids on plant growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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