Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1971 Feb;47(2):282–288. doi: 10.1104/pp.47.2.282

The Respiratory Chain of Plant Mitochondria

IX. Oxidation-Reduction Potentials of the Cytochromes of Mung Bean Mitochondria

P Leslie Dutton a, Bayard T Storey a
PMCID: PMC365855  PMID: 16657609

Abstract

The oxidation-reduction potentials of the cytochromes of the respiratory chain of mung bean (Phaseolus aureus) mitochondria have been measured under strictly anaerobic conditions with a combined spectrophotometric/potentiometric method. The midpoint potentials at pH 7.2 are as follows: cytochrome a: +190 millivolts; a3: +380 millivolts; b553: +75 millivolts; b557: +42 millivolts; b562: −77 millivolts; c547 and c549: +235 millivolts. (The subscripts refer to the difference absorbance maxima observed for these cytochromes in reduced-minus-oxidized difference spectra recorded at 77 K.) The same values of midpoint potentials at pH 7.2 are obtained with mitochondria depleted of energy by aerobic incubation with ADP and uncoupler in the presence of inorganic phosphate, or with coupled mitochondria energized with ATP in the absence of inorganic phosphate. Coupling site II is placed between b553/b557 and c549/c547 in these mitochondria, and coupling site III is placed between a and a3.

Full text

PDF
282

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K., WILSON T. H. The free-energy changes for the reduction of diphosphopyridine nucleotide and the dehydrogenation of L-malate and L-glycerol 1-phosphate. Biochem J. 1953 Apr;54(1):86–94. doi: 10.1042/bj0540086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. D., Jr, Plesnicar M. Electron transport carriers in plant mitochondria. Nature. 1967 May 6;214(5088):616–617. doi: 10.1038/214616a0. [DOI] [PubMed] [Google Scholar]
  3. CHANCE B., HOLLUNGER G. The interaction of energy and electron transfer reactions in mitochondria. VI. The efficiency of the reaction. J Biol Chem. 1961 May;236:1577–1584. [PubMed] [Google Scholar]
  4. CHANCE B., HOLMES W., HIGGINS J., CONNELLY C. M. Localization of interaction sites in multi-component transfer systems: theorems derived from analogues. Nature. 1958 Nov 1;182(4644):1190–1193. doi: 10.1038/1821190a0. [DOI] [PubMed] [Google Scholar]
  5. CHANCE B. The interaction of energy and electron transfer reactions in mitochondria. II. General properties of adenosine triphosphate-linked oxidation of cytochrome and reduction of pyridine nucleotide. J Biol Chem. 1961 May;236:1544–1554. [PubMed] [Google Scholar]
  6. CHANCE B. The interaction of energy and electron transfer reactions in mitochondria. V. The energy transfer pathway. J Biol Chem. 1961 May;236:1569–1576. [PubMed] [Google Scholar]
  7. Chance B., Wilson D. F., Dutton P. L., Erecińska M. Energy-coupling mechanisms in mitochondria: kinetic, spectroscopic, and thermodynamic properties of an energy-transducing form of cytochrome b. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1175–1182. doi: 10.1073/pnas.66.4.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Erecinska M., Storey B. T. The Respiratory Chain of Plant Mitochondria: VII. Kinetics of Flavoprotein Oxidation in Skunk Cabbage Mitochondria. Plant Physiol. 1970 Oct;46(4):618–624. doi: 10.1104/pp.46.4.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ikuma H., Bonner W. D. Properties of Higher Plant Mitochondria. I. Isolation and Some Characteristics of Tightly-coupled Mitochondria from Dark-grown Mung Bean Hypocotyls. Plant Physiol. 1967 Jan;42(1):67–75. doi: 10.1104/pp.42.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KLINGENBERG M., SCHOLLMEYER P. ATP controlled redox states of respiratory carriers under the influence of DPNH-hydrogen accepting substrates. Biochem Biophys Res Commun. 1961 Apr 7;4:323–327. doi: 10.1016/0006-291x(61)90211-x. [DOI] [PubMed] [Google Scholar]
  11. Lance C., Bonner W. D. The respiratory chain components of higher plant mitochondria. Plant Physiol. 1968 May;43(5):756–766. doi: 10.1104/pp.43.5.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Storey B. T., Bahr J. T. The respiratory chain of plant mitochondria. I. Electron transport between succinate and oxygen in skunk cabbage mitochondria. Plant Physiol. 1969 Jan;44(1):115–125. doi: 10.1104/pp.44.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Storey B. T. Chemical hypothesis for energy conservation in the mitochondrial respiratory chain. J Theor Biol. 1970 Aug;28(2):233–259. doi: 10.1016/0022-5193(70)90054-8. [DOI] [PubMed] [Google Scholar]
  14. Storey B. T. The Respiratory Chain of Plant Mitochondria. III. Oxidation Rates of the Cytochromes c and b in Mung Bean Mitochondria Reduced With Succinate. Plant Physiol. 1969 Mar;44(3):413–421. doi: 10.1104/pp.44.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Storey B. T. The Respiratory Chain of Plant Mitochondria: VIII. Reduction Kinetics of the Respiratory Chain Carriers of Mung Bean Mitochondria with Reduced Nicotinamide Adenine Dinucleotide. Plant Physiol. 1970 Oct;46(4):625–630. doi: 10.1104/pp.46.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Storey B. T. The respiratory chain of plant mitochondria. IV. Oxidation rates of the respiratory carriers of mung bean mitochondria in the presence of cyanide. Plant Physiol. 1970 Apr;45(4):447–454. doi: 10.1104/pp.45.4.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Storey B. T. The respiratory chain of plant mitochondria. V. Reaction of reduced cytochromes a and a3 in mung bean mitochondria with oxygen in the presence of cyanide. Plant Physiol. 1970 Apr;45(4):455–460. doi: 10.1104/pp.45.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Urban P. F., Klingenberg M. On the redox potentials of ubiquinone and cytochrome b in the respiratory chain. Eur J Biochem. 1969 Jul;9(4):519–525. doi: 10.1111/j.1432-1033.1969.tb00640.x. [DOI] [PubMed] [Google Scholar]
  19. Wilson D. F., Chance B. Azide inhibition of mitochondrial electron transport. I. The aerobic steady state of succinate oxidation. Biochim Biophys Acta. 1967 May 9;131(3):421–430. doi: 10.1016/0005-2728(67)90002-3. [DOI] [PubMed] [Google Scholar]
  20. Wilson D. F., Dutton P. L. Energy dependent changes in the oxidation-reduction potential of cytochrome b. Biochem Biophys Res Commun. 1970 Apr 8;39(1):59–64. doi: 10.1016/0006-291x(70)90757-6. [DOI] [PubMed] [Google Scholar]
  21. Wilson D. F., Dutton P. L. The oxidation-reduction potentials of cytochromes a and a3 in intact rat liver mitochondria. Arch Biochem Biophys. 1970 Feb;136(2):583–585. doi: 10.1016/0003-9861(70)90233-x. [DOI] [PubMed] [Google Scholar]
  22. Wohlrab H. Equilibration of reducing equivalents among the terminal portions of the mitochondrial respiratory chains. Biochemistry. 1970 Feb 3;9(3):474–479. doi: 10.1021/bi00805a004. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES