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. 1971 Mar;47(3):335–341. doi: 10.1104/pp.47.3.335

Induction of Coleoptile Elongation by Carbon Dioxide 1

Michael L Evans a,2, Peter M Ray a,3, Leonora Reinhold a,4
PMCID: PMC365865  PMID: 16657618

Abstract

The ability of CO2 to induce elongation of Avena sativa coleoptile segments was examined with the use of a high resolution growth-recording device. CO2-saturated water causes an 8- to 16-fold promotion in the rate of elongation within 1 minute. This elongation is insensitive to a variety of metabolic inhibitors that suppress auxin-induced elongation, and the CO2 effect cannot be prevented by pretreatment with these inhibitors. Buffers of pH 3 to 4 also stimulate elongation quickly, and it seems that at least a major part of the action of CO2 depends upon its ability to reduce pH. The rate of elongation of auxin-promoted segments can be further enhanced by treatment with CO2 but not vice versa.

The response to CO2 can be inhibited by mannitol at osmotic concentrations that inhibit normal growth, by calcium, and by brief pretreatment with heavy water (D2O). The elongation rate that results from CO2 treatment is sensitive to temperature, but the induction by CO2 itself appears to be almost temperature-independent.

Elongation following treatment with CO2 may be a physical flow phenomenon, essentially independent of immediate biochemical participation, which occurs when wall polymer interactions that normally restrict strain in the cell wall are weakened or broken by CO2 in a manner that in effect substitutes for the role of metabolism in normal auxin-inducible cell enlargement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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