Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1971 Mar;47(3):400–403. doi: 10.1104/pp.47.3.400

Kinetin Inhibition of 3H-Uracil and 14C-Leucine Incorporation by Tobacco Cells in Suspension Culture

Uri Nudel 1, Elchanan S Bamberger 1
PMCID: PMC365877  PMID: 16657630

Abstract

The rate of 14C-leucine and 3H-uracil incorporation by tobacco cells (Nicotiana tabaccum var. Samsun N.N.) in suspension culture was simultaneously decreased by the addition of kinetin at concentrations above 2.5 × 10−5m. Ribosomal RNA was the first RNA species affected by kinetin. The purine derivatives, adenine and N6-methyl-aminopurine, which exhibit low cytokinin activity overcame the inhibitory effects of kinetin. However, purine derivatives without cytokinin activity, guanine, N6,6-dimethyl-aminopurine, and 2-aminopurine, did not relieve kinetin inhibition.

Full text

PDF
400

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhattacharyya J., Roy S. C. Growth promoters and the synthesis of protein in plant mitochondria. 1. Effect of kinetin on the incorporation of amino acids into mitochondrial protein. Biochem Biophys Res Commun. 1969 Jun 6;35(5):606–610. doi: 10.1016/0006-291x(69)90447-1. [DOI] [PubMed] [Google Scholar]
  2. Click R. E., Hackett D. P. The isolation of ribonucleic acid from plant, bacterial or animal cells. Biochim Biophys Acta. 1966 Oct 24;129(1):74–84. doi: 10.1016/0005-2787(66)90010-4. [DOI] [PubMed] [Google Scholar]
  3. Datta A., Sen S. P. The mechanism of action of plant growth substances: growth substance stimulation of amino acid incorporation into nuclear protein. Biochim Biophys Acta. 1965 Sep 13;107(2):352–357. doi: 10.1016/0304-4165(65)90141-8. [DOI] [PubMed] [Google Scholar]
  4. HURWITZ J., FURTH J. J., MALAMY M., ALEXANDER M. The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1222–1230. doi: 10.1073/pnas.48.7.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Helgeson J. P. The cytokinins. Synthetic and naturally occurring N6-substituted adenine derivatives profoundly affect plant growth. Science. 1968 Sep 6;161(3845):974–981. doi: 10.1126/science.161.3845.974. [DOI] [PubMed] [Google Scholar]
  6. Kemp H. T., Fuller R. G., Davidson R. S. Inhibition of Plant Growth by Root-Drench Applications of Kinetin. Science. 1957 Dec 6;126(3284):1182–1182. doi: 10.1126/science.126.3284.1182. [DOI] [PubMed] [Google Scholar]
  7. Klämbt D., Thies G., Skoog F. Isolation of cytokinins from Corynebacterium fascians. Proc Natl Acad Sci U S A. 1966 Jul;56(1):52–59. doi: 10.1073/pnas.56.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leonard N. J., Hecht S. M., Skoog F., Schmitz R. Y. Cytokinins: synthesis, mass spectra, and biological activity of compounds related to zeatin. Proc Natl Acad Sci U S A. 1969 May;63(1):175–182. doi: 10.1073/pnas.63.1.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  10. Osborne D. J. Effect of Kinetin on Protein & Nucleic Acid Metabolism in Xanthium Leaves During Senescence. Plant Physiol. 1962 Sep;37(5):595–602. doi: 10.1104/pp.37.5.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roychoudhury R., Datta A., Sen S. P. The mechanism of action of plant growth substances: the role of nuclear RNA in growth substance action. Biochim Biophys Acta. 1965 Sep 13;107(2):346–351. doi: 10.1016/0304-4165(65)90140-6. [DOI] [PubMed] [Google Scholar]
  12. SKOOG F., MILLER C. O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol. 1957;11:118–130. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES