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. 1972 Jan;49(1):52–57. doi: 10.1104/pp.49.1.52

Regulation of Aromatic Amino Acid Biosynthesis in Higher Plants

I. Evidence for a Regulatory Form of Chorismate Mutase in Etiolated Mung Bean Seedlings 1

D G Gilchrist a,2, Terry S Woodin a,3, Marilyn L Johnson a, T Kosuge a
PMCID: PMC365900  PMID: 16657896

Abstract

Etiolated mung bean seedlings were examined for chorismate mutase activity. Evidence for the occurrence of two forms of the enzyme (designated CM-1 and CM-2) was obtained by ammonium sulfate fractionation, anion exchange cellulose chromatography, and isoelectric focusing. The two forms showed distinctly different properties, as CM-1 was inhibited by phenylalanine and tyrosine and activated by tryptophan, but inhibition by phenylalanine and tyrosine was reversed by tryptophan. The other form, CM-2, was unaffected by any of the three aromatic amino acids. Isoelectric points of the two forms were CM-1, pH 4.6, and CM-2, pH 5.6. The molecular weights estimated by molecular sieving on Sephadex G-200 were CM-1, 50,000, and CM-2, 36,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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