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. 1972 Feb;49(2):207–211. doi: 10.1104/pp.49.2.207

Subcellular Localization of Zinc and Calcium in Bean (Phaseolus vulgaris L.) Tissues 1

V S Rathore a,2, Y P S Bajaj a,3, S H Wittwer a
PMCID: PMC365930  PMID: 16657926

Abstract

Two bean (Phaseolus vulgaris L.) cultivars differing in growth responses to zinc were examined for differences in uptake and subcellular localization of 65Zn during a 15-day growth period. The zinc-sensitive cultivar Sanilac showed initially a much higher rate of absorption, which declined after 24 hours. The zinc-tolerant cultivar Saginaw showed a slow but steady rate of absorption for 10 days. In roots as well as in stem callus tissues of both cultivars, three-fourths of the absorbed 65Zn was localized in the “cytoplasmic” supernatant fractions (containing ribosomes and vacuolar sap). Very little (less than 7%) 65Zn was localized in the cell wall fraction. There was a much greater proportion of the absorbed 65Zn localized in root mitochondria and nuclei of the zinc-sensitive Sanilac than in the zinc-tolerant Saginaw. Stem callus tissues, however, did not show such cultivar differences in zinc accumulation at the sub-cellular level.

Calcium-45 distribution in the cultivar Redkote showed preferential affinity for cell walls both in roots and stem cellus tissue. The percentage of absorbed 45Ca associated with cell wall fractions of roots gradually increased with a corresponding decline in the percentage of radioactivity associated with the cytoplasmic fraction. At 15 days, 57% of the total 45Ca in the roots was localized in the cell walls. A similar, albeit less pronounced, preferential 45Ca sorption on cell walls occurred for the callus tissue. Calcium-45 also accumulated at later stages in the nuclear fraction, but there was no mitochondrial accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benedict C. R., Beevers H. Formation of sucrose from malate in germinating castor beans. I. Conversion of malate to phosphoenol-pyruvate. Plant Physiol. 1961 Sep;36(5):540–544. doi: 10.1104/pp.36.5.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandon P. C. Artifacts in intracellular enzyme distribution caused by alkaline homogenates. Plant Physiol. 1969 Mar;44(3):461–462. doi: 10.1104/pp.44.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burling E., Jackson W. T. Changes in Calcium Levels in Cell Walls during Elongation of Oat Coleoptile Sections. Plant Physiol. 1965 Jan;40(1):138–141. doi: 10.1104/pp.40.1.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dalgarno L., Birt L. M. Aspects of malate metabolism by slices and mitochondria from carrot tissue. Biochem J. 1962 Apr;83(1):202–211. doi: 10.1042/bj0830202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EDWARDS C., OLSON K. B., HEGGEN G., GLENN J. Intracellular distribution of trace elements in liver tissue. Proc Soc Exp Biol Med. 1961 May;107:94–97. doi: 10.3181/00379727-107-26546. [DOI] [PubMed] [Google Scholar]
  7. Gielink A. J., Sauer G., Ringoet A. Histoautoradiographic localization of calcium in oat plant tissues. Stain Technol. 1966 Sep;41(5):281–286. doi: 10.3109/10520296609116323. [DOI] [PubMed] [Google Scholar]
  8. Heber U., Pon N. G., Heber M. Localization of Carboxydismutase & Triosephosphate Dehydrogenases in Chloroplasts. Plant Physiol. 1963 May;38(3):355–360. doi: 10.1104/pp.38.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MEDINA A., NICHOLAS D. J. Some properties of a zinc-dependent hexokinase from Neurospora crassa. Biochem J. 1957 Aug;66(4):573–578. doi: 10.1042/bj0660573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mayer A. M. Subcellular location of sulphite reductase in plant tissues. Plant Physiol. 1967 Mar;42(3):324–326. doi: 10.1104/pp.42.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NASON A., KAPLAN N. O., OLDEWURTEL H. A. Further studies of nutritional conditions affecting enzymatic constitution in Neurospora. J Biol Chem. 1953 Mar;201(1):435–444. [PubMed] [Google Scholar]
  12. Ritenour G. L., Joy K. W., Bunning J., Hageman R. H. Intracellular localization of nitrate reductase, nitrite reductase, and glutamic Acid dehydrogenase in green leaf tissue. Plant Physiol. 1967 Feb;42(2):233–237. doi: 10.1104/pp.42.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. THIERS R. E., VALLEE B. L. Distribution of metals in subcellular fractions of rat liver. J Biol Chem. 1957 Jun;226(2):911–920. [PubMed] [Google Scholar]
  14. Whatley F. R., Ordin L., Arnon D. I. DISTRIBUTION OF MICRONUTRIENT METALS IN LEAVES AND CHLOROPLAST FRAGMENTS. Plant Physiol. 1951 Apr;26(2):414–418. doi: 10.1104/pp.26.2.414. [DOI] [PMC free article] [PubMed] [Google Scholar]

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