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. 1972 Mar;49(3):376–380. doi: 10.1104/pp.49.3.376

Inhibition of Chloroplast Reactions with Phenylmercuric Acetate 1,2

Richard C Honeycutt a,3, David W Krogmann a
PMCID: PMC365968  PMID: 16657964

Abstract

Phenylmercuric acetate is a selective inhibitor of the photosynthetic activities of isolated spinach (Spinacia oleracea) chloroplasts. At 5 μm concentration of phenylmercuric acetate, photophosphorylation is inhibited. At 33 μm phenylmercuric acetate, ferredoxin is inactivated. Ferredoxin-NADP oxidoreductase is 50% inhibited at 100 μm phenylmercuric acetate. Photosystem II reactions are 50% inhibited at 150 μm phenylmercuric acetate and very much higher cooncentrations—500 μm—are needed to approach complete inhibition. Phenylmercuric acetate inhibition of photosystem II appears to be selective, blocking a site between the 3-(3,4-dichlorophenyl)-1,1-dimethyl urea sensitive site and the site inactivated by high concentrations of tris buffer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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