Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1972 Apr;49(4):482–489. doi: 10.1104/pp.49.4.482

The Influence of Axis Removal on Protein Metabolism in Cotyledons of Pisum sativum L. 1

T Y Chin a,2, Rozanne Poulson a,3, Leonard Beevers a,4
PMCID: PMC365992  PMID: 16657988

Abstract

The protein metabolism of cotyledons attached to the embryonic axis has been compared with that in cotyledons removed from the axis at the initiation of a 6-day imbibition. Total protein declined in the attached but not in the detached cotyledons. Concurrent with the decline in protein level in the intact cotyledons there was an increased capacity to incorporate exogenously supplied leucine into protein. In contrast, detached cotyledons showed a restricted capacity for protein synthesis. It was demonstrated that ribosomal preparations from cotyledons of intact seedlings contained an increasing proportion of polyribosomes as germination progressed and such ribosomes were active in in vitro amino acid incorporation. Ribosomal preparations from detached cotyledons contained few polyribosomes and had a restricted capacity to incorporate amino acids in vitro. The in vitro incorporation of phenylalanine was stimulated by polyuridylic acid with the stimulation being greatest in ribosomal preparations from detached cotyledons. The results suggest that an axis component may regulate the availability of messenger RNA in the cotyledons during germination.

Full text

PDF
482

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barker G. R., Hollinshead J. A. The degradation of ribonucleic acid in the cotyledons of Pisum arvense. Biochem J. 1967 Apr;103(1):230–237. doi: 10.1042/bj1030230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breidenbach R. W., Castelfranco P., Criddle R. S. Biogenesis of Mitochondria in Germinating Peanut Cotyledons II. Changes in Cytochromes and Mitochondrial DNA. Plant Physiol. 1967 Aug;42(8):1035–1041. doi: 10.1104/pp.42.8.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cherry J. H. Nucleic Acid, Mitochondria, & Enzyme Changes in Cotyledons of Peanut Seeds during Germination. Plant Physiol. 1963 Jul;38(4):440–446. doi: 10.1104/pp.38.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hewish D. R., Wheldrake J. F., Wells J. R. Incorporation of 32P into ribosomal RNA, transfer RNA and inositol hexaphosphate in germinating pea cotyledons. Biochim Biophys Acta. 1971 Jan 28;228(2):509–516. doi: 10.1016/0005-2787(71)90056-6. [DOI] [PubMed] [Google Scholar]
  5. Ingle J., Hageman R. H. Metabolic Changes Associated with the Germination of Corn. III. Effects of Gibberellic Acid on Endosperm Metabolism. Plant Physiol. 1965 Jul;40(4):672–675. doi: 10.1104/pp.40.4.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jachymczyk W. J., Cherry J. H. Studies on messenger RNA from peanut plants: in vitro polyribosome formation and protein synthesis. Biochim Biophys Acta. 1968 Apr 22;157(2):368–377. doi: 10.1016/0005-2787(68)90091-9. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
  9. MARCUS A., FEELEY J. PROTEIN SYNTHESIS IN IMBIBED SEEDS. II. POLYSOME FORMATION DURING IMBIBITION. J Biol Chem. 1965 Apr;240:1675–1680. [PubMed] [Google Scholar]
  10. Paleg L. G. Physiological Effects of Gibberellic Acid: I. On Carbohydrate Metabolism and Amylase Activity of Barley Endosperm. Plant Physiol. 1960 May;35(3):293–299. doi: 10.1104/pp.35.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Penner D., Ashton F. M. Hormonal control of proteinase activity in squash cotyledons. Plant Physiol. 1967 Jun;42(6):791–796. doi: 10.1104/pp.42.6.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ross C., Coddington R. L., Murray M. G., Bledsoe C. S. Pyrimidine metabolism in cotyledons of germinating alaska peas. Plant Physiol. 1971 Jan;47(1):71–75. doi: 10.1104/pp.47.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Varner J. E., Balce L. V., Huang R. C. Senescence of Cotyledons of Germinating Peas. Influence of Axis Tissue. Plant Physiol. 1963 Jan;38(1):89–92. doi: 10.1104/pp.38.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES