Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1972 May;49(5):779–782. doi: 10.1104/pp.49.5.779

Induction of Phenylalanine Ammonia Lyase and Pisatin by Photosensitive Psoralen Compounds 1

Lee A Hadwiger a
PMCID: PMC366051  PMID: 16658047

Abstract

The psoralen compounds, xanthotoxin and 4,5′, 8-trimethylpsoralen, when activated, increased phenylalanine ammonia lyase (PAL) activity and the synthesis of pisatin in excised pea pods. Pods presoaked 1 hr with 4,5′,8-trimethylpsoralen and then irradiated 4 minutes with 366 nanometer ultraviolet light had twice as much PAL activity 3 hours after irradiation and 12 times as much PAL activity 20 hours after irradiation as the pods of the water-treated control. Increases in PAL activity and pisatin synthesis were not obtained with 4,5′,8-trimethylpsoralen, xanthotoxin, or 366 nanometer light treatment alone. 4,5′,8-Trimethylpsoralen in combination with the irradiation treatment (366 nanometers) enhanced the rate at which l-leucine is incorporated into various fractions of soluble proteins in excised pods 8 hours after treatment. This treatment decreased the rate at which orotic acid is incorporated into RNA. The increase in PAL activity induced by irradiated psoralens was prevented when 6-methylpurine (0.5 milligram per milliliter) or cycloheximide (10 micrograms per milliliter) was applied immediately following the irradiation period. Possible functions of psoralen compounds in plants are discussed.

Full text

PDF
779

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cole R. S. Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells. Biochim Biophys Acta. 1970 Sep 17;217(1):30–39. doi: 10.1016/0005-2787(70)90119-x. [DOI] [PubMed] [Google Scholar]
  2. Drake J. W., McGuire J. Properties of r mutants of bacteriophage T4 photodynamically induced in the presence of thiopyronin and psoralen. J Virol. 1967 Apr;1(2):260–267. doi: 10.1128/jvi.1.2.260-267.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hadwiger L. A., Schwochau M. E. Specificity of deoxyribonucleic Acid intercalating compounds in the control of phenylalanine ammonia lyase and pisatin levels. Plant Physiol. 1971 Mar;47(3):346–351. doi: 10.1104/pp.47.3.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MUSAJO L., RODIGHIERO G. The skin-photosensitizing furocoumarins. Experientia. 1962 Apr 15;18:153–161. doi: 10.1007/BF02151700. [DOI] [PubMed] [Google Scholar]
  5. Musajo L., Bordin F., Caporale G., Marciani S., Rigatti G. Photoreactions at 3655 Angstrom between pyrimidine bases and skin-photosensitizing furocoumarins. Photochem Photobiol. 1967 Oct;6(10):711–719. doi: 10.1111/j.1751-1097.1967.tb08736.x. [DOI] [PubMed] [Google Scholar]
  6. Musajo L., Rodighiero G., Breccia A., Dall'Acqua F., Malesani G. Skin-photosensitizing furocoumarins: photochemical interaction between DNA and -O14CH3 bergapten (5-methoxy-psoralen). Photochem Photobiol. 1966 Sep;5(9):739–745. doi: 10.1111/j.1751-1097.1966.tb05823.x. [DOI] [PubMed] [Google Scholar]
  7. OGINSKY E. L., GREEN G. S., GRIFFITH D. G., FOWLKS W. L. Lethal photosensitization of bacteria with 8-methoxypsoralen to long wave length ultraviolet radiation. J Bacteriol. 1959 Dec;78:821–833. doi: 10.1128/jb.78.6.821-833.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  9. POLLOCK M. R. THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D ON THE BIOSYNTHESIS OF ENZYMES IN BACILLUS SUBTILIS AND BACILLUS CEREUS. Biochim Biophys Acta. 1963 Sep 17;76:80–93. [PubMed] [Google Scholar]
  10. Pathak M. A., Krämer D. M. Photosensitization of skin in vivo by furocoumarins (psoralens). Biochim Biophys Acta. 1969 Nov 19;195(1):197–206. doi: 10.1016/0005-2787(69)90616-9. [DOI] [PubMed] [Google Scholar]
  11. REPPEL L. Uber natürliche Cumarine. Pharmazie. 1954 Apr;9(4):278–299. [PubMed] [Google Scholar]
  12. Rodighiero G., Chandra P., Wacker A. Structural specificity for the photoinactivation of nucleic acids by furocoumarins. FEBS Lett. 1970 Sep 18;10(1):29–32. doi: 10.1016/0014-5793(70)80408-2. [DOI] [PubMed] [Google Scholar]
  13. SCHEEL L. D., PERONE V. B., LARKIN R. L., KUPEL R. E. THE ISOLATION AND CHARACTERIA CHARACTERIZATION OF TWO PHOTOTOXIC FURANOCOUMARINS (PSORALENS) FROM DISEASED CELERY. Biochemistry. 1963 Sep-Oct;2:1127–1131. doi: 10.1021/bi00905a038. [DOI] [PubMed] [Google Scholar]
  14. Schwochau M. E., Hadwiger L. A. Regulation of gene expression by actinomycin D and other compounds which change the conformation of DNA. Arch Biochem Biophys. 1969 Oct;134(1):34–41. doi: 10.1016/0003-9861(69)90247-1. [DOI] [PubMed] [Google Scholar]
  15. Schwochau M. E., Hadwiger L. A. Stimulation of pisatin production in Pisum sativum by actinomycin D and other compounds. Arch Biochem Biophys. 1968 Aug;126(2):731–733. doi: 10.1016/0003-9861(68)90463-3. [DOI] [PubMed] [Google Scholar]
  16. Szendrei K., Novák I., Varga E., Buzás G. Uber die Wirkstoffe von Dictamnus albus L. 1. Photodynamische Stoffe. Pharmazie. 1968 Feb;23(2):76–77. [PubMed] [Google Scholar]
  17. Waring M. J. Drugs which affect the structure and function of DNA. Nature. 1968 Sep 28;219(5161):1320–1325. doi: 10.1038/2191320a0. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES