Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1972 May;49(5):848–851. doi: 10.1104/pp.49.5.848

Cytokinins in Pisum Transfer Ribonucleic Acid 1

Hendrik J Vreman a, Folke Skoog a, Charles R Frihart b, Nelson J Leonard b
PMCID: PMC366063  PMID: 16658059

Abstract

Five cytokinin-active ribonucleosides have been isolated from the transfer RNA of 7-day-old green pea shoots (Pisum sativum L. var. Alaska). Ultraviolet spectroscopy and mass spectrometry have been used to identify 6-(3-methyl-2-butenylamino)-9-β-d-ribofuranosylpurine, 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-β- d-ribofuranosylpurine, and 6-(4-hydroxy-3-methyl-2-butenylamino)-9-β-d-ribofuranosylpurine. The latter was separated into the cis- and trans-isomers by thin layer chromatography. The fifth cytokinin is indicated to be 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-d -ribofuranosylpurine on the basis of its chromatographic properties.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. J., Burrows W. J., Skoog F., Roy K. L., Söll D. Cytokinins: distribution in transfer RNA species of Escherichia coli. Proc Natl Acad Sci U S A. 1969 Jul;63(3):834–841. doi: 10.1073/pnas.63.3.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong D. J., Evans P. K., Burrows W. J., Skoog F., Petit J. F., Dahl J. L., Steward T., Strominger J. L., Leonard N. J., Hecht S. M. Cytokinins. Activity and identification in Staphylococcus epidermidis transfer ribonucleic acid. J Biol Chem. 1970 Jun 10;245(11):2922–2926. [PubMed] [Google Scholar]
  3. Armstrong D. J., Skoog F., Kirkegaard L. H., Hampel A. E., Bock R. M., Gillam I., Tener G. M. Cytokinins: distribution in species of yeast transfer RNA. Proc Natl Acad Sci U S A. 1969 Jun;63(2):504–511. doi: 10.1073/pnas.63.2.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Babcock D. F., Morris R. O. Quantitative measurement of isoprenoid nuceosides in transfer ribonucleic acid. Biochemistry. 1970 Sep 15;9(19):3701–3705. doi: 10.1021/bi00821a008. [DOI] [PubMed] [Google Scholar]
  5. Bartz J., Söll D., Burrows W. J., Skoog F. Identification of the cytokinin-active ribonucleosides in pure Escherichia coli tRNA species. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1448–1453. doi: 10.1073/pnas.67.3.1448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burrows W. J., Armstrong D. J., Kaminek M., Skoog F., Bock R. M., Hecht S. M., Dammann L. G., Leonard N. J., Occolowitz J. Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. Biochemistry. 1970 Apr 28;9(9):1867–1872. doi: 10.1021/bi00811a001. [DOI] [PubMed] [Google Scholar]
  7. Burrows W. J., Armstrong D. J., Skoog F., Hecht S. M., Boyle J. T., Leonard N. J., Occolowitz J. The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids. Biochemistry. 1969 Jul;8(7):3071–3076. doi: 10.1021/bi00835a057. [DOI] [PubMed] [Google Scholar]
  8. Burrows W. J., Skoog F., Leonard N. J. Isolation and identification of cytokinins located in the transfer ribonucleic acid of tobacco callus grown in the presence of 6-benzylaminopurine. Biochemistry. 1971 Jun 8;10(12):2189–2194. doi: 10.1021/bi00788a002. [DOI] [PubMed] [Google Scholar]
  9. Hall R. H., Csonka L., David H., McLennan B. Cytokinins in the soluble RNA of plant tissues. Science. 1967 Apr 7;156(3771):69–71. doi: 10.1126/science.156.3771.69. [DOI] [PubMed] [Google Scholar]
  10. Hall R. H., Srivastava I. S. Cytokinin activity of compounds obtained from soluble RNA. Life Sci. 1968 Jan 15;7(2):7–13. doi: 10.1016/0024-3205(68)90341-x. [DOI] [PubMed] [Google Scholar]
  11. Hecht S. M., Leonard N. J., Burrows W. J., Armstrong D. J., Skoog F., Occolowitz J. Cytokinin of wheat germ transfer RNA: 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine. Science. 1969 Dec 5;166(3910):1272–1274. doi: 10.1126/science.166.3910.1272. [DOI] [PubMed] [Google Scholar]
  12. Letham D. S., Ralph R. K. A cytokinin in soluble RNA from a higher plant. Life Sci. 1967 Feb 15;6(4):387–394. doi: 10.1016/0024-3205(67)90008-2. [DOI] [PubMed] [Google Scholar]
  13. Playtis A. J., Leonard N. J. The synthesis of ribosyl-cis-zeatin and thin layer chromatographic separation of the cis and trans isomers of ribosylzeatin. Biochem Biophys Res Commun. 1971 Oct 1;45(1):1–5. doi: 10.1016/0006-291x(71)90041-6. [DOI] [PubMed] [Google Scholar]
  14. Robins M. J., Hall R. H., Thedford R. N-6-(delta-3-isopentenyl) adenosine. A component of the transfer ribonucleic acid of yeast and of mammalian tissue, methods of isolation, and characterization. Biochemistry. 1967 Jun;6(6):1837–1848. doi: 10.1021/bi00858a035. [DOI] [PubMed] [Google Scholar]
  15. Skoog F., Armstrong D. J., Cherayil J. D., Hampel A. E., Bock R. M. Cytokinin activity: localization in transfer RNA preparations. Science. 1966 Dec 9;154(3754):1354–1356. doi: 10.1126/science.154.3754.1354. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES