Figure 4. MiR-146a-deficient lymphocytes contribute to the HSC defect and myeloproliferation.
(A)–(M) Age-and-sex-matched WT, miR-146a KO (miR KO), Rag1 KO and miR-146a/Rag1 double KO (miR/Rag1 DKO) mice were allowed to age to 10-month-old before harvested for analysis. (A) Representative FACS plots of CD150+CD48− HSCs of the LSK gate. Quantification of total number of HSCs (B), LSK cells (C), and Lin−cKit+Sca1− myeloid progenitor cells (D) in BM. Quantification of total number of CD45+ (E), CD11b+ (F), and Ter119+ (G) cells in BM. (H) Representative histological pictures (H&E stain) of femur bones. Scale bar, 40 μm. (I). Representative photograph of spleens and spleen weight. Total number of CD45+ (J), CD11b+ (K), and Gr1+ (L) cells in spleen. (M) For comparison, various cell lineages in spleen of only Rag1 KO and miR/Rag1 DKO mice were regraphed from (J–L).