Abstract
On the basis of polyacrylamide gradient gel electrophoresis of leaf extracts from 24 species of higher plants, two main forms of carbonic anhydrase (EC 4.2.1.1) were recognized; the “dicotyledon” type and the “monocotyledon” type. More than one band of enzyme was found on gels from most species, suggesting the possibility of carbonic anhydrase isoenzymes in higher plants.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atkins C. A., Patterson B. D., Graham D. Plant Carbonic Anhydrases: II. Preparation and Some Properties of Monocotyledon and Dicotyledon Enzyme Types. Plant Physiol. 1972 Aug;50(2):218–223. doi: 10.1104/pp.50.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowes G. W. Carbonic anhydrase in marine algae. Plant Physiol. 1969 May;44(5):726–732. doi: 10.1104/pp.44.5.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brewer J. M. Artifact produced in disc electrophoresis by ammonium persulfate. Science. 1967 Apr 14;156(3772):256–257. doi: 10.1126/science.156.3772.256. [DOI] [PubMed] [Google Scholar]
- Headings V. E., Tashian R. E. Isoenzymes of carbonic anhydrase I from primate red blood cells. Biochim Biophys Acta. 1971 Apr 27;236(1):353–359. doi: 10.1016/0005-2795(71)90185-1. [DOI] [PubMed] [Google Scholar]
- Maren T. H. Carbonic anhydrase: chemistry, physiology, and inhibition. Physiol Rev. 1967 Oct;47(4):595–781. doi: 10.1152/physrev.1967.47.4.595. [DOI] [PubMed] [Google Scholar]
- Margolis J., Kenrick K. G. Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Anal Biochem. 1968 Oct 24;25(1):347–362. doi: 10.1016/0003-2697(68)90109-7. [DOI] [PubMed] [Google Scholar]
- Mitchell W. M. A potential source of electrophoretic artifacts in polyacrylamide gels. Biochim Biophys Acta. 1967 Sep 19;147(1):171–174. doi: 10.1016/0005-2795(67)90101-8. [DOI] [PubMed] [Google Scholar]
- Patterson B. D., Atkins C. A., Graham D., Wills R. B. Carbonic anhydrase: a new method of detection on polyacrylamide gels using low-temperature fluorescence. Anal Biochem. 1971 Dec;44(2):388–391. doi: 10.1016/0003-2697(71)90224-7. [DOI] [PubMed] [Google Scholar]
- RICKLI E. E., GHAZANFAR S. A., GIBBONS B. H., EDSALL J. T. CARBONIC ANHYDRASES FROM HUMAN ERYTHROCYTES. PREPARATION AND PROPERTIES OF TWO ENZYMES. J Biol Chem. 1964 Apr;239:1065–1078. [PubMed] [Google Scholar]
- Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. doi: 10.1073/pnas.46.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TASHIAN R. E. GENETIC VARIATION AND EVOLUTION OF THE CARBOXYLIC ESTERASES AND CARBONIC ANHYDRASES OF PRIMATE ERYTHROCYTES. Am J Hum Genet. 1965 May;17:257–272. [PMC free article] [PubMed] [Google Scholar]
- Tashian R. E., Shreffler D. C., Shows T. B. Genetic and phylogenetic variation in the different molecular forms of mammalian erythrocyte carbonic anhydrases. Ann N Y Acad Sci. 1968 Jun 14;151(1):64–77. doi: 10.1111/j.1749-6632.1968.tb11878.x. [DOI] [PubMed] [Google Scholar]
- Tobin A. J. Carbonic anhydrase from parsley leaves. J Biol Chem. 1970 May 25;245(10):2656–2666. [PubMed] [Google Scholar]
- Wistrand P. J., Rao S. N. Immunologic and kinetic properties of carbonic anhydrases from various tissues. Biochim Biophys Acta. 1968 Jan 22;154(1):130–144. doi: 10.1016/0005-2795(68)90265-1. [DOI] [PubMed] [Google Scholar]