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. 1972 Oct;50(4):446–451. doi: 10.1104/pp.50.4.446

The Activity of Ribulose Diphosphate Carboxylase in Extracts of Gonyaulax polyedra in the Day and the Night Phases of the Circadian Rhythm of Photosynthesis 1

Karen J Bush a,2, Beatrice M Sweeney a
PMCID: PMC366166  PMID: 16658193

Abstract

The ribulose 1,5-diphosphate carboxylase from Gonyaulax polyedra Stein. has a half-life of about four hours in buffer, but can be stabilized by the addition of 50% glycerol. The optimum pH is 7.8 to 8.0 and the optimum Mg2+ concentration is 3 mm. Heavy metal ions (Cu2+, Hg2+, Ni2+, Zn2+), EDTA, pyrophosphate, and adenosine triphosphate were strongly inhibitory. Ribulose 1,5-diphosphate carboxylase from Gonyaulax was not cold-sensitive or activated by light activation factor from tomato or Gonyaulax. No difference in the activity of this enzyme was detected when extracts prepared at the maximum and the minimum of the circadian rhythm of photosynthesis were compared. The Km of HCO3− was also the same (16 to 19 mm).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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