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. 1972 Oct;50(4):463–468. doi: 10.1104/pp.50.4.463

Cytochemical Localization of Catalase in Glyoxysomes Isolated from Maize Scutella

Giovanna P Longo a, Carola Dragonetti a, Claudio P Longo a
PMCID: PMC366169  PMID: 16658196

Abstract

The localization of catalase in isolated maize scutellum glyoxysomes was investigated by means of the diaminobenzidine histochemical reaction. Only the membranes of the glyoxysomes become heavily stained after incubation with diaminobenzidine and H2O2. If the glyoxysomes are lysed with Tricine buffer at pH 9, 70% of the catalase is solubilized, while the remaining 30% is tightly bound to an insoluble fraction formed mostly by glyoxysomal membranes. This suggests that catalase may be present also in the matrix of the glyoxysomes. The lack of staining of the matrix with diaminobenzidine is probably due to the high concentration of catalase in the membranes of the organelles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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