Abstract
Particulate preparations from Phaseolus aureus produce a d-mannosyl-lipid when treated with GDP-d-mannose. This lipid complex appears to be an active d-mannose donor, and some investigators have proposed that its role might be an obligatory intermediate in mannan synthesis of higher plants. When the partially purified d-mannosyl-lipids, isotopically labeled in the d-mannose moiety, were treated with particulate enzymes under a variety of conditions, a negligible amount of material was produced that behaved as a polysaccharide. Endogenous, particle-bound d-mannosyl-14C-lipid prepared from P. aureus particles readily transferred d-mannose to GDP to yield GDP-d-mannose and was hydrolyzed to free d-mannose when treated briefly with 0.01 n HCl at 100 C. The d-mannosyl-lipid, therefore, exhibits active d-mannose transfer potential in its endogenous state. When endogenous glycosyl-lipid was incubated in the absence of GDP-d-mannose-14C, little or no polysaccharide was produced. It was, instead, slowly degraded to d-mannose. Addition of several different unlabeled sugar nucleotides had no effect on the results. Our studies to date, therefore, offer no evidence that the mannosyl-lipid is an obligatory precursor of polysaccharide.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANDERSON J. S., MATSUHASHI M., HASKIN M. A., STROMINGER J. L. LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE: PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS. Proc Natl Acad Sci U S A. 1965 Apr;53:881–889. doi: 10.1073/pnas.53.4.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ASPINALL G. O. Structural chemistry of the hemicelluloses. Adv Carbohydr Chem. 1959;14:429–468. doi: 10.1016/s0096-5332(08)60228-3. [DOI] [PubMed] [Google Scholar]
- BANDURSKI R. S., AXELROD B. The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951 Nov;193(1):405–410. [PubMed] [Google Scholar]
- BANGHAM A. D., DAWSON R. M. The relation between the activity of a lecithinase and the electrophoretic charge of the substrate. Biochem J. 1959 Jul;72:486–492. doi: 10.1042/bj0720486. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
- Elbein A. D. Biosynthesis of a cell wall glucomannan in mung bean seedlings. J Biol Chem. 1969 Mar 25;244(6):1608–1616. [PubMed] [Google Scholar]
- Elbein A. D., Hassid W. Z. The enzymatic synthesis of a glucomannan. Biochem Biophys Res Commun. 1966 May 3;23(3):311–318. doi: 10.1016/0006-291x(66)90547-x. [DOI] [PubMed] [Google Scholar]
- Kauss H. A plant mannosyl-lipid acting in reversible transfer of mannose. FEBS Lett. 1969 Sep;5(1):81–84. doi: 10.1016/0014-5793(69)80298-x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Liu T. Y., Hassid W. Z. Solubilization and partial purification of cellulose synthetase from Phaseolus aureus. J Biol Chem. 1970 Apr 25;245(8):1922–1925. [PubMed] [Google Scholar]
- Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scher M., Lennarz W. J. Studies on the biosynthesis of mannan in Micrococcus lysodeikticus. I. Characterization of mannan-14C formed enzymatically from mannosyl-1-phosphoryl-undecaprenol. J Biol Chem. 1969 May 25;244(10):2777–2789. [PubMed] [Google Scholar]
- Troy F. A., Frerman F. E., Heath E. C. The biosynthesis of capsular polysaccharide in Aerobacter aerogenes. J Biol Chem. 1971 Jan 10;246(1):118–133. [PubMed] [Google Scholar]
- Villemez C. L. Characterization of intermediates in plant cell wall biosynthesis. Biochem Biophys Res Commun. 1970 Aug 11;40(3):636–641. doi: 10.1016/0006-291x(70)90951-4. [DOI] [PubMed] [Google Scholar]
- Villemez C. L., Clark A. F. A particle bound intermediate in the biosynthesis of plant cell wall polysaccharides. Biochem Biophys Res Commun. 1969 Jul 7;36(1):57–63. doi: 10.1016/0006-291x(69)90649-4. [DOI] [PubMed] [Google Scholar]
- Villemez C. L. Rate studies of polysaccharide biosynthesis from guanosine diphosphate -D-glucose and guanosine diphosphate -D-mannose. Biochem J. 1971 Jan;121(1):151–157. doi: 10.1042/bj1210151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiner I. M., Higuchi T., Rothfield L., Saltmarsh-Andrew M., Osborn M. J., Horecker B. L. Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium. Proc Natl Acad Sci U S A. 1965 Jul;54(1):228–235. doi: 10.1073/pnas.54.1.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright A., Dankert M., Robbins P. W. Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen. Proc Natl Acad Sci U S A. 1965 Jul;54(1):235–241. doi: 10.1073/pnas.54.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]