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. 1972 Oct;50(4):473–476. doi: 10.1104/pp.50.4.473

The Role of a d-Mannosyl-Lipid as an Intermediate in the Synthesis of Polysaccharide in Phaseolus aureus Seedlings 1

David L Storm a, W Z Hassid a
PMCID: PMC366171  PMID: 16658198

Abstract

Particulate preparations from Phaseolus aureus produce a d-mannosyl-lipid when treated with GDP-d-mannose. This lipid complex appears to be an active d-mannose donor, and some investigators have proposed that its role might be an obligatory intermediate in mannan synthesis of higher plants. When the partially purified d-mannosyl-lipids, isotopically labeled in the d-mannose moiety, were treated with particulate enzymes under a variety of conditions, a negligible amount of material was produced that behaved as a polysaccharide. Endogenous, particle-bound d-mannosyl-14C-lipid prepared from P. aureus particles readily transferred d-mannose to GDP to yield GDP-d-mannose and was hydrolyzed to free d-mannose when treated briefly with 0.01 n HCl at 100 C. The d-mannosyl-lipid, therefore, exhibits active d-mannose transfer potential in its endogenous state. When endogenous glycosyl-lipid was incubated in the absence of GDP-d-mannose-14C, little or no polysaccharide was produced. It was, instead, slowly degraded to d-mannose. Addition of several different unlabeled sugar nucleotides had no effect on the results. Our studies to date, therefore, offer no evidence that the mannosyl-lipid is an obligatory precursor of polysaccharide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON J. S., MATSUHASHI M., HASKIN M. A., STROMINGER J. L. LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE: PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS. Proc Natl Acad Sci U S A. 1965 Apr;53:881–889. doi: 10.1073/pnas.53.4.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ASPINALL G. O. Structural chemistry of the hemicelluloses. Adv Carbohydr Chem. 1959;14:429–468. doi: 10.1016/s0096-5332(08)60228-3. [DOI] [PubMed] [Google Scholar]
  3. BANDURSKI R. S., AXELROD B. The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951 Nov;193(1):405–410. [PubMed] [Google Scholar]
  4. BANGHAM A. D., DAWSON R. M. The relation between the activity of a lecithinase and the electrophoretic charge of the substrate. Biochem J. 1959 Jul;72:486–492. doi: 10.1042/bj0720486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
  6. Elbein A. D. Biosynthesis of a cell wall glucomannan in mung bean seedlings. J Biol Chem. 1969 Mar 25;244(6):1608–1616. [PubMed] [Google Scholar]
  7. Elbein A. D., Hassid W. Z. The enzymatic synthesis of a glucomannan. Biochem Biophys Res Commun. 1966 May 3;23(3):311–318. doi: 10.1016/0006-291x(66)90547-x. [DOI] [PubMed] [Google Scholar]
  8. Kauss H. A plant mannosyl-lipid acting in reversible transfer of mannose. FEBS Lett. 1969 Sep;5(1):81–84. doi: 10.1016/0014-5793(69)80298-x. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Liu T. Y., Hassid W. Z. Solubilization and partial purification of cellulose synthetase from Phaseolus aureus. J Biol Chem. 1970 Apr 25;245(8):1922–1925. [PubMed] [Google Scholar]
  11. Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scher M., Lennarz W. J. Studies on the biosynthesis of mannan in Micrococcus lysodeikticus. I. Characterization of mannan-14C formed enzymatically from mannosyl-1-phosphoryl-undecaprenol. J Biol Chem. 1969 May 25;244(10):2777–2789. [PubMed] [Google Scholar]
  13. Troy F. A., Frerman F. E., Heath E. C. The biosynthesis of capsular polysaccharide in Aerobacter aerogenes. J Biol Chem. 1971 Jan 10;246(1):118–133. [PubMed] [Google Scholar]
  14. Villemez C. L. Characterization of intermediates in plant cell wall biosynthesis. Biochem Biophys Res Commun. 1970 Aug 11;40(3):636–641. doi: 10.1016/0006-291x(70)90951-4. [DOI] [PubMed] [Google Scholar]
  15. Villemez C. L., Clark A. F. A particle bound intermediate in the biosynthesis of plant cell wall polysaccharides. Biochem Biophys Res Commun. 1969 Jul 7;36(1):57–63. doi: 10.1016/0006-291x(69)90649-4. [DOI] [PubMed] [Google Scholar]
  16. Villemez C. L. Rate studies of polysaccharide biosynthesis from guanosine diphosphate -D-glucose and guanosine diphosphate -D-mannose. Biochem J. 1971 Jan;121(1):151–157. doi: 10.1042/bj1210151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weiner I. M., Higuchi T., Rothfield L., Saltmarsh-Andrew M., Osborn M. J., Horecker B. L. Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium. Proc Natl Acad Sci U S A. 1965 Jul;54(1):228–235. doi: 10.1073/pnas.54.1.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wright A., Dankert M., Robbins P. W. Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen. Proc Natl Acad Sci U S A. 1965 Jul;54(1):235–241. doi: 10.1073/pnas.54.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]

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