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. 1972 Nov;50(5):541–546. doi: 10.1104/pp.50.5.541

Isolation and Characterization of Membranes from the Cultivated Mushroom 1

R Barry Holtz a,2, Peter S Stewart a, Stuart Patton a, Lee C Schisler b
PMCID: PMC366186  PMID: 16658213

Abstract

The membranes of sporophore cap tissue from the cultivated mushroom, Agaricus bisporus (Lange) Sing., were isolated using discontinuous sucrose gradient ultracentrifugation of a tissue homogenate. A membrane-rich fraction was concentrated at the 1.16/1.18 g/cc interface and a mitochondria-rich fraction at the 1.18/1.20 g/cc interface. The membrane fraction was judged to be greater than 90% membrane vesicles by electron microscopy. The protein to lipid ratio of the membrane fraction was 1.1; the molar ratio of sterol to phospholipid was 0.77. The specific radioactivity of a Mg-activated ATPase was 2.5 times greater in the membrane fraction than in the homogenate. No 5′-nucleotidase or Na-K-Mg-activated ATPase activity was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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