Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1972 Nov;50(5):627–631. doi: 10.1104/pp.50.5.627

Immunological Comparisons of Chymotrypsin Inhibitor I among Several Genera of the Solanaceae 1

S Gurusiddaiah a,2, T Kuo a, C A Ryan a,3
PMCID: PMC366203  PMID: 16658230

Abstract

Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae were identified by enzymatic assay. In leaves of three genera, Solanum, Lycopersicum, and Datura, chymotrypsin inhibitor I was identified immunologically. In petals of all seven genera inhibitor I was also identified immunologically. With the microcomplement fixation technique inhibitor I from leaf or petal extracts of eight Solanaceae genera were compared. An immunological relationship of inhibitor I among seven of these genera was established.

Full text

PDF
627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALLS A. K., RYAN C. A. CONCERNING A CRYSTALLINE CHYMOTRYPTIC INHIBITOR FROM POTATOES, AND ITS BINDING CAPACITY FOR THE ENZYME. J Biol Chem. 1963 Sep;238:2976–2982. [PubMed] [Google Scholar]
  2. Green T. R., Ryan C. A. Wound-Induced Proteinase Inhibitor in Plant Leaves: A Possible Defense Mechanism against Insects. Science. 1972 Feb 18;175(4023):776–777. doi: 10.1126/science.175.4023.776. [DOI] [PubMed] [Google Scholar]
  3. HUMMEL B. C. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol. 1959 Dec;37:1393–1399. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Melville J. C., Ryan C. A. Chymotrypsin inhibitor I from potatoes. Large scale preparation and characterization of its subunit components. J Biol Chem. 1972 Jun 10;247(11):3445–3453. [PubMed] [Google Scholar]
  6. Prager E. M., Wilson A. C. The dependence of immunological cross-reactivity upon sequence resemblance among lysozymes. II. Comparison of precipitin and micro-complement fixation results. J Biol Chem. 1971 Nov 25;246(22):7010–7017. [PubMed] [Google Scholar]
  7. Ryan C. A. An inducible protein in potato and tomato leaflets. Plant Physiol. 1968 Nov;43(11):1880–1881. doi: 10.1104/pp.43.11.1880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ryan C. A., Huisman O. C., Van Denburgh R. W. Transitory Aspects of a Single Protein in Tissues of Solanum tuberosum and Its Coincidence With the Establishment of New Growth. Plant Physiol. 1968 Apr;43(4):589–596. doi: 10.1104/pp.43.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ryan C. A. Synthesis of chymotrypsin inhibitor I protein in potato leaflets induced by detachment. Plant Physiol. 1968 Nov;43(11):1859–1865. doi: 10.1104/pp.43.11.1859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sarich V. M., Wilson A. C. Immunological time scale for hominid evolution. Science. 1967 Dec 1;158(3805):1200–1203. doi: 10.1126/science.158.3805.1200. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES